This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (Luc-CAG-Tat/SPI-CD79α-CD28-Myc) and CRISPR-Cas (Cas12b (C2c1)-RNA-2xHEPN-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | SPM754 |
Host | Mouse |
Targeting Cell Type | Ramos (RA 1) |
Reporter gene | Luc |
Promoter | CAG |
Signal peptides | Tat/SPI |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | Myc |
Linkers | SEG |
Co-Receptors | LILRB |
Vector Type | AAV |
Vector Name | BGpCDH1 |
Class | 2 single-Cas |
Type | IV |
Subtype | B |
Effector | Cas12b (C2c1) |
DNA Type | RNA |
Nuclease domains | 2xHEPN |
TracrRNA requirement | No |
PAM/PFS | NNGRRT |
Insertion | Wap |
Construction | Luc-CAG-CD79α-Cas12b (C2c1)-RNA-Wap |
Target | S100A1 |
Alternative Names | S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1 |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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