S100A1 mAb (S-1/61) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR21)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-MNDU3-Sec/SPII-CD79α-CD28-CBP) and CRISPR-Cas (Cas13a (C2c2)-ssRNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone S-1/61
Host Mouse
Targeting Cell Type Raji-Luc2
Reporter gene GFP
Promoter MNDU3
Signal peptides Sec/SPII
Signaling domains CD79α
TM domains CD28
Detection tags CBP
Linkers Glycine-Serine linker
Co-Receptors CD22
Vector Type VSV
Vector Name BGpCDH7
Class 1 multi-Cas
Type I
Subtype A, B
Effector Cas13a (C2c2)
DNA Type ssRNA
Nuclease domains HD/Cas3
TracrRNA requirement No
PAM/PFS 5'PFS: non-C; 3'PFS:NAN/NNA
Insertion Stat5b
Construction GFP-MNDU3-CD79α-Cas13a (C2c2)-ssRNA-Stat5b

Target Information

Target S100A1
Alternative Names S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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