Cas No.: 129179-83-5
PRODUCT INFORMATION
Description
Biotin-HPDP is a thiol-reactive, cleavable biotinylation reagent. It features a 2-pyridyldithiol (HPDP) group that reacts specifically with free sulfhydryl groups (cysteines) to form a disulfide bond. This linkage is stable under normal conditions but is readily cleaved by reducing agents (e.g., DTT, TCEP). This reagent is used to biotinylate cysteine-containing proteins or peptides. Its key application is in affinity purification, where the biotinylated "bait" protein and its interacting partners can be captured on streptavidin and then gently released by cleaving the disulfide bond.
Molecular Weight
539.77
Formula
C₂₄H₃₇N₅O₃S₃
Product Type
Biotins and Streptavidins
Group 1
Biotin
Group 2
Other
Form (20 deg.C)
Solid
Storage Condition
Store under inert gas, -20°C
Molecular Structure
For Research Use Only | Not For Clinical Use
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