CPO-PFP Selective N-Terminal Cysteine Residue Crosslinker (CAT#: OLY-1023-YS4)

Description

CPO-PFP is a highly specialized crosslinker for the site-specific modification of N-terminal cysteine (N-Cys) residues. It features a highly reactive pentafluorophenyl (PFP) active ester and a cyclopropene (CPO) group. The PFP ester first acylates the alpha-amine of the N-terminus. This tethers the linker, positioning the CPO moiety for a rapid intramolecular reaction with the N-Cys thiol. This proximity-driven mechanism grants exceptional selectivity for N-terminal cysteines over internal cysteine residues, making it invaluable for creating homogenous, site-specific protein conjugates or for peptide stapling.

Molecular Weight

306.19

Purity

>98.0%

Formula

C₁₃H₇F₅O₃

Product Type

Heterobifunctional Crosslinkers ; Selective N-Terminal Cysteine Residue Conjugation Reagents

Group 1

Other

Group 2

Other

Form (20 deg.C)

Solid

Storage Condition

Store under inert gas, -20°C

Molecular Structure