As a preeminent contract antibody development company, Creative Biolabs offers high-quality in vitro diagnostic (IVD) antibody development services targeting a wide range of biomarkers. Here, we focus on CD154 marker of heart failure and rheumatoid arthritis. We are committed to providing the highest quality of custom services and products at the most reasonable prices.
CD154, also known as gp39, T-BAM, TRAP and Ly-62, is a CD40 ligand that belongs to the TNF family. It is a 39 kDa type II membrane glycoprotein firstly reported to be restricted to activate CD40 positive helper T lymphocytes. There are two forms of CD154 in lymphocytes: a cell bound full-length form (CD154, 30 kDa) and a truncated soluble form (sCD154, 18 kDa). The CD154 homotrimer is formed on many cell types such as basophils, B lymphocytes, activated T lymphocytes and so on. The CD40 receptor was initially authenticated and functionally characterized on B lymphocytes and acts a crucial role as an adjuster of T-cell-dependent B cell activation, proliferation and differentiation. The extensive spectrum of CD40–CD154 expression has developed a novel role of the CD40–CD154 dyad as a pivotal mediator of immunity and inflammation. It has indicated that the CD40–CD154 axis up regulates proinflammatory mediators, for example, the formation of proinflammatory cytokines (i.e. TNF-a, IL-6). These mediators have been proven to be increased in chronic heart failure (CHF), and possibly contribute to the development and progression of CHF.
Figure 1. CD154 and its receptors. CD154 (CD40L) is a member of the TNF superfamily, mostly expressed on activated CD4+ T lymphocytes but is discovered in a soluble form as well. CD40-CD154-mediated contact-dependent signals between B and T cells are needed for the formation of thymus dependent (TD) humoral immune responses. The interactions between CD154 and CD40 are very essential in a number of other cell systems. (Hassan, G. S. 2013)
CD154 Marker of Heart Failure
CHF is a chronic progressive situation that influences the pumping capacity of heart muscles, particularly intends to the stage in which fluid builds up around the heart and results in inefficiently pumping of heart. Inflammation acts an important conducive role in the pathogenesis of CHF. Increased plasma levels of proinflammatory cytokines were discovered in patients with CHF, such as TNF-α and IL-6, as well as a persistent immune activation. CD154 serves a crucial role in the pathophysiology of multicellular vascular events, including inflammation, thrombosis, atherosclerosis, etc. Researches have proven that there is a notably raised level of CD154 on platelets in patients with CHF. The result displayed that the CD154 is available to be used as a potential marker for diagnosis of CHF.
Figure 2. This schematic reveals the regulation of platelet CD40L, as well as the role of sCD40L in signaling after binding to platelet CD40 and αIIbβ3 causing an auto-amplification loop. (Aloui, C. 2014)
CD154 Marker of Rheumatoid Arthritis
RA is a long-lasting inflammatory disorder that mainly affects joints. However, sometimes it also damages a great number of body systems, such as the skin, lungs, eyes, heart and blood vessels. Ligation of activated CD154+ CD4+ T cells to CD40 expressed on endothelial cells results in enhancing leucocyte margination and diapedesis due to the upregulation of some adhesion molecules. A study has revealed that improved expression of CD154 on CD4+ T cells in RA displayed prolonged and raised activation of CD4+ T lymphocytes and is related to active disease and probably an adverse prognosis.
IVD Antibody Development Services Targeting CD154 Marker
IVD antibodies have been widely used in immunodiagnostic tools to aid in the diagnosis of various diseases, conditions, and infections. With years of experience and advanced technology, Creative Biolabs is able to provide a full range of high-quality and cost-effective biomarker-specific antibody development services. Especially, we help develop novel anti-CD154 antibodies for your IVD projects. Having completed a number of IVD antibody generation and development projects, Creative Biolabs is confident to offer you the best products and services to promote your project a success.
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