As a leading CRO of antibody generation and development, Creative Biolabs offers application-specific antibody development services to global clients. With experienced scientists and advanced technology, we have established a series of high-quality in vitro diagnostic (IVD) antibody development services against biomarkers of different diseases. Here, we introduce our IVD antibody development services for NAG marker.
N-acetyl-β-(D)-glucosaminidase (NAG) is a lysosomal enzyme of proximal renal tubular cells. It functions as an acidic hydrolase which hydrolyzes N-acetyl-β-D-glucosidase linkage existed in glycoprotein and lipoprotein. NAG is a macromolecular protein and the molecular weight is about 140,000. It almost distributes in all tissues, including prostate and kidney. Especially in the proximal renal tubular lysosome, it has the highest concentration. The NAG has many isoenzymes, and type A and B isoenzymes are the major form of existence in most tissues. Certainly, there are trace amounts of I1, I2S, and P isozymes in a few tissues. There are three kinds of isoenzymes, NAG-A, NAG-B and NAG-I in kidney tissue.
Fig. 1 The structure of N-acetyl-β-(D)-glucosaminidase.
NAG Marker of Acute Kidney Injury (AKI)
AKI is sudden episode of kidney failure or kidney damage that happens within a few hours or a few days. Decreased kidney blood flow, exposure to substances harmful to the kidney and an inflammatory process in the kidney may lead to AKI occurrence. AKI is a complex clinical syndrome which is difficult to predict and identify in the early period.
Most studies have been shown that urinary NAG may serve as an early detection and prognostic marker in AKI. NAG is a lysosomal enzyme which is major located in the proximal tubular epithelial cell. Due to its large molecular weight, it can not be filtered by glomerular. Normally, NAG activity in the renal tissue, especially in renal tubular epithelial cells is much higher than the ureter and lower urethra. However, when the kidney suffers from damage and injury, NAG will leak into urine, resulting in increased NAG activity in the urine. Hence, urinary NAG activity may reflect renal parenchymal lesions. And an increase in the urinary activity of NAG is a sensitive and reasonably specific measure of AKI. Besides, the urinary NAG activity is increased in chronic nephritis, chronic renal failure, lupus nephritis, nephrotic syndrome, rejection after renal transplantation, toxic nephropathy, epidemic hemorrhagic fever, late cirrhosis and so on.
IVD Antibody Development Services Targeting NAG Marker
Aided by our advanced IVD platform, Creative Biolabs is able to generate antibodies with high affinity and specificity that are specific to diagnostic targets, such as NAG marker. Besides, we also provide services to biotech companies and academic researchers for their needs in developing diagnostic immunoassays of various formats, including lateral flow assay, ELISA, latex-based assays, etc. With the successful completion of numerous projects, Creative Biolabs is confident in providing services and products of the best quality.
If you are interested in the services we provide, please don't hesitate to contact us or directly send us an inquiry. We will get in touch with you as soon as possible.
For lab research use only.