Microarray can provide genomic and proteomic information for several biological targets at the same time, which is a significant progress because they provide more complete and reliable information and simplify the whole analysis scheme. Creative Biolabs provides this microsphere-based multiplex immunoassay for simultaneous detection of protein and DNA in a single test.
Microsphere assays have become an important form of multiple analysis such as genotyping, gene expression and protein immunoassay. Different microsensors can be analyzed simultaneously by chemical, spectrometric or physical means to encode each type of probe functionalized microspheres. A rolling circle amplification (RCA) microarray based on high-density microspheres has been developed for simultaneous detection of protein and DNA in a single test. With this new method of detecting proteins and protein related target DNA, medical conditions can be diagnosed by a rapid analysis, rather than using different tests to detect different disease markers.
RCA is an alternative amplification method for improving the sensitivity of DNA quantification, DNA mutation detection and array-based sandwich immunoassay. Due to its strict requirements for the matching of the stringent strands, RCA is a simple, highly sensitive and specific amplification method that can produce high-efficiency amplification.
RCA products contain thousands of repeat sequences that complement fluorescent labeled signal probes. Because RCA can be used as a signal generation method for antibody detection, it can also be compatible with the sandwich immunoassay format. The integrity of the antibody-antigen complex was maintained during isothermal amplification, and the amplified DNA products could be covalently or connected to the detection antibody through the avidin biotin bridge.
Fig.1 Microsphere-based RCA assay for the detection of a target DNA sequence and IL-8. (Konry, 2009)
Our microsphere-based multiplex immunoassay platform can realize the combined detection of nucleic acid sequences and proteins in one detection. This assay only generates a signal when both analytes are present simultaneously. Due to the high amplification efficiency of RCA and the high binding capacity of the microsphere surface, the detection limit of protein and DNA can reach fM and pM levels, respectively.
By collecting a wide range of catalog and non-catalog nucleic acids, antibodies and proteins, our team can quickly assess the feasibility of the target and add it to the existing microsphere array, ultimately reducing your time to obtain data and minimizing the sample size required. Our customized services include array optimization, microsphere modification and customized analysis and development.
If you are interested in our service, please contact us for your exclusive solution.
Reference
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