Liquid Array Diagnostics

The increasing number of proteomics, transcriptomics and genomic sequence data obtained from public databases provides a rare opportunity for the development of new and diversified detection technologies. At present, the technology based on the concept of liquid (suspension) array has been established and widely used in pharmaceutical, clinical and research laboratories. Creative Biolabs provides professional method development and detection services based on liquid array diagnostics (LAD) technology.

Introduction of LAD

Unlike the conventional microarray technology, which is characterized by the position of the analyte on the slide, LAD technology uses different groups of microspheres in liquid suspension as the determinant of analyte specificity. The microsphere groups are internally stained with two kinds of fluorophores with different spectra. The spectral characteristics are unique for each microsphere and are determined by the different concentrations of the internal dyes, resulting in a microsphere array of 100 members with different spectra. The integration of a third internal dye has allowed the expansion of microspheres with up to 500 members.

The surface of each microsphere group allows simple chemical coupling of various reagents specific to a specific bioassay, such as nucleic acid assay, immunoassay, enzyme assay or receptor-ligand assay. Another fluorescent reporter (e.g., streptavidin-r-phycoerythrin, Alexa 532, Cy3) is coupled to the target molecule, which allowed specific capture on the surface of the microspheres for detection.

Fig.1 The LAD technology based on internally dyed microspheres. (Reslova, 2017)

Methods of Aptamer Pair Development in Creative Biolabs

There are different types of microsphere available, and their selection is usually determined by the type of instrument used for detection and the specific target analyte. Microsphere-based technology can be used in a variety of analytical forms. According to the type of analyte, we divide them into:

• Microsphere-based multiplex immunoassay
• Microsphere-based nucleic acid assays
• Microsphere-based protein assays

In general, compared with other commonly used methods, the LAD based analysis format is very open and flexible, ensuring that the result data can be obtained in a few hours with a very small number of samples, and the experimental variability can be minimized due to the use of multiple data points from a single experiment.

Fig.2 An overview of LAD method. (Hiseni, 2019)

What Can We Do?

• Sufficient experience: rich project experience to enhance our service confidence;
• Excellent quality control: the test in the production process through a number of quality control steps to ensure long-term reproducibility and consistency;
• Specificity: for each target analyte, all target analytes were screened;
• Custom service: professional customization team can help you meet your unique needs.

If you are interested in our service, please contact us for your exclusive solution.

References

1. Reslova, N.; et al. xMAP technology: applications in detection of pathogens. Frontiers in microbiology. 2017, 8: 55.
2. Hiseni, P.; et al. Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions. BioTechniques. 2019, 66(3): 143-149.

For Research Use Only.