As a leading provider of superior antibody development services, Creative Biolabs provides a complete portfolio of antibody services, including antibody stability design, allosteric effect analysis, de novo design, modular antibody design, and more. our expert staff is available to assist you with every phase of antibody development.
Allostery is a regulated biological process by which the effect of binding of macromolecules at one site is transmitted to another site. Although monoclonal antibodies (mAbs) are highly specific in terms of biological activity, they also exhibit allosteric effects. These allosteric effects can result in changes in the conformation of the antigen, thereby affecting its biological activity and interaction with other mAbs. In general, the allosteric effects of antibodies can be divided into two types: negative allosteric effects and positive allosteric effects.
Negative allosteric effects
Positive allosteric effects
mAbs are widely used as biological tools due to their invariable specificity. However, the interpretation of results can be misled by the behaviour of mAbs displaying allosteric effects. Some mAbs have been shown to modify the spatial structure of the corresponding protein antigen, affecting its biological activity as well as its binding to a second mAb. In this way, the negative allosteric effect on the conformation affects the binding of other mAbs. Thus, a researcher using a mAb as a tool to investigate some features of an antigenic molecule must be aware of the possible negative allosteric properties of the antibody.
In some cases, allosteric increases the affinity of antibodies for the epitopes they bind to. In addition, several studies have revealed the positive effects of allosteric mAbs on various diseases. For example, to develop a novel treatment for patients with type 2 diabetes, Bhaskar et al. used an allosteric, human mAb called XMetA and applied phage display technology to target insulin receptors coupled with insulin. In this study, XMetA showed allosteric effects and markedly reduced elevated fasting blood glucose and normalized glucose tolerance. Therefore, allosteric mAbs may be novel, ultra-long-acting agents that regulate diabetes and hyperglycemia.
Over the years, Creative Biolabs has developed a number of methods to study antibody allosteric effects and its applications in different scientific fields, such as conformational mechanisms, drug discovery, and various diseases. We provide extensive antibody allosteric effect analytical services to help our clients get an in-depth understanding of their mAbs candidates in terms of their physicochemical properties, bioactivity, affinity, and stability. We can analyze the antibody structure as a starting point to solve a series of problems such as the theoretical design of an antibody.
With our professional knowledge and advanced technology, Creative Biolabs is confident to provide the most comprehensive antibody analysis and design services to satisfy your specific needs. If you have any questions, please feel free to contact us and our experienced technicians will give you the most detailed answers to your questions.
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