Computational antibody discovery has made it possible to generate novel opportunities to understand the fundamental principles of molecular recognition, and to develop effective antibodies for research, and for diagnostic and therapeutic applications. Creative Biolabs is dedicated to establishing the most exquisite service platform for our clients and the antibody design services can provide comprehensive technical support for advancing our clients’ projects.
Modular design is well suited to address the challenge of designing antibodies with a range of different biophysical properties. The design strategies implemented on modular platforms involve a peptide-design and grafting procedure, which, in synergy with an accurate prediction of solubility, enables the design of novel antibodies targeting linear epitopes of interest.
The CamSol method of predicting protein solubility was employed to reduce the tendency of the antibody scaffold to self-associate, as well as to rank different designed paratopes to select highly soluble binding regions. The optimization of antibody solubility is a crucial requirement for a successful antibody design strategy, as antibodies perform their function in crowded molecular environments, and need to interact specifically with their target while avoiding non-specific interactions and self-association, in particular under storage conditions.
The Cascade method was used to design the antibody paratope. This method enables the design of linear motifs, called complementary peptides, which are predicted to bind to a target epitope and are grafted onto the CDR of an antibody scaffold. The Cascade procedure starts by collecting from the PDB all fragments facing in a b-strand any subsequence of the target epitope of at least three residues. Then, starting from the longest of these fragments, complementary peptides are grown to the length of the target epitope by joining some of the other fragments.
Fig. 1 Illustration of the rational design strategy of modular.
In silico rational modular design has the potential of becoming the preferred procedure for the generation of antibodies for many applications, as this approach offers unique opportunities to accelerate discoveries in the biomedical sciences. It promises not only to provide a cheaper and faster route to antibody discovery but also to enable uniquely precise control over the properties of the resulting antibodies. We can offer modular design services to meet specific customer requirements.
Creative Biolabs has focused on the development of antibody discovery for years, we whole-heartedly cooperate with you to accomplish our shared goals. Our team provides you with outstanding support and meets your specific needs with a professional technology platform. If you are interested in our services, please feel free to contact us or directly sent us an inquiry.
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