Antisense RNA is a single-stranded RNA that is complementary to “sense” mRNA sequence strand to inhibit gene expression in many different organisms including plants, flies, worms, and fungi. Creative Biolabs provides antisense RNA synthesis service to turn off target genes. This screening method highly aids target identification process.

Messenger RNA (mRNA) is a single-stranded molecule that is used as the template for protein translation. The second sequence of RNA (nonsense RNA) complementary to the mRNA is possible to form duplexes and inhibits translation of mRNA as it physically obstructing the translation machinery. This second sequence is called antisense RNA.

The RNAi process is initiated by double-stranded RNA fragments, and this double helix is the actual suppressor of its corresponding gene. Using RNAi screen services allows you to obtain the most suitable antisense RNA.

Antisense RNA is a single strand RNA. When it is introduced into a cell, the regulation involves three classes of mechanisms:

  1. Antisense RNAs directly bind to the coding region of sense RNA, resulting in direct inhibition of translation or mRNA destabilization.

Figure 1. Antisense RNA mediated regulatory mechanisms (Brantl 2002) Figure 1. Antisense RNA mediated regulatory mechanisms (Brantl 2002)

  1. RNA duplex transcripts may mask trans-acting factors which are critical for regulating transcription, like mRNA splicing, transport, polyadenylation, translation, and degradation.
  2. The formation of double-stranded RNAs may affect gene expression via RNA editing or RNAi gene slicing. When antisense RNA is introduced into cells, it binds to existent sense RNA by forming an RNA double helix. Then gene suppression occurred not only by inhibiting transcription of existing mRNA but also by following-up RNA interference (RNAi) as described.

Creative Biolabs uses antisense RNA inhibition in the identification and validation of essential genes to accelerate your drug discovery process. This technique has also been used to confer fungal resistance to antifungal drugs. We have a patent technology for cloning vectors containing promoters for RNA polymerases. It makes in vitro synthesis of single-stranded RNA molecules routinely and quickly. Besides plasmid DNA, PCR products can be used as templates for transcription reactions. The primers of PCR is specially designed which include the promoter sequence of the polymerase. The high yields of RNA transcripts of our platform are especially suitable for the synthesis of large mass amounts of RNA.

Introducing of antisense RNA can also be achieved by introducing a gene encoding the antisense RNA. It can be achieved by using a plasmid vector or using a gene gun that shoots microscopic tungsten pellets coated with the gene. This is quicker and easier than creating a knockout fungus for target gene studying. Using antisense RNA as a tool in this way is an exciting prospect for many molecular biologists.

Antisense RNA emerges as a promising approach to identify potential novel therapeutic targets. Creative Biolabs is confident in offering high-quality antisense RNA service. Our researchers will formulate systematic plans and perform precisely tailored protocols in order to facilitate our customers' programs best.

For more detailed information, please feel free to contact us or directly sent us an inquiry.

Reference

  1. Brantl S (2002). “Antisense-RNA regulation and RNA interference”. Biochimica et Biophysica Acta 1575: 15-25.

For Research Use Only.


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