Neurotoxicity Evaluation by 3D Neuronal Spheroids

Creative Biolabs offers neurotoxicity evaluation services based on the 3D neuronal spheroid model, providing more accurate and reliable data for drug discovery. This helps customers gain a better understanding of the safety and efficacy of neuro pharmaceuticals, thereby expediting the drug development process.

Comprehensive Explanation of the 3D Neuronal Spheroid Model

The 3D neuronal spheroid is an in vitro cell model formed by the natural aggregation of neuronal cells into a three-dimensional spherical structure under conditions without a matrix or with the addition of hydrogels. This model holds significant importance for neurobiological research and drug discovery.

Analysis of images for 3D neural spheroids originated from human iPSCs. Fig 1. Image analysis of human
iPSC-derived 3D neural spheroids.¹

Compared to traditional 2D cell culture models and animal models, the 3D neuronal spheroid model exhibits the following advantages:

Increased Physiological Relevance	Facilitates High-Throughput Analysis
The 3D neuronal spheroid model can mimic the physiological environment of neural cells in vivo, including cell-cell interactions, cell-matrix interactions, cell differentiation, and morphogenesis processes.	Employing high-content imaging analysis systems enables automated imaging and analysis of large-scale 3D neuronal spheroid models, enhancing efficiency and accuracy.

3D Neuronal Spheroid Model Construction Process

Cell Selection and Preparation

Select appropriate neural cells as the study subjects, often using iPSC-derived neurons or other types of neural cells.

Cultivate Neuronal Spheroids

Utilize suitable 3D culture techniques and cell culture plates or scaffolds to allow cells to naturally aggregate and form 3D cellular spheroids under conditions without a matrix or with the addition of hydrogels.

Compound or Drug Addition

Once the cellular spheroids are formed, the test compounds or drugs can be added to the culture medium, exposing the spheroids to different concentrations of the substances.

Comprehensive Performance Testing

Cell Viability Testing	Calcium Oscillation Assay
Use appropriate cell viability assay kits to assess the impact of different compound concentrations on neuronal cell viability.	Calcium ions play a vital role in signal transduction in neuronal cells. This test monitors the dynamic changes of calcium ions within the neuronal spheroids to evaluate their response.
Staining and Imaging	Patch-Clamp Recording
Perform immunofluorescence and immunohistochemistry on treated 3D cellular spheroids, using high-resolution microscopy or high-content imaging systems. These systems can capture images at different depths within the spheroids.	By forming patch-clamp recordings on the neuronal spheroids, cell electrophysiological parameters such as membrane potential and ion channel activity can be measured.
Sequencing and Expression Analysis	Multi-Electrode Array (MEA) Testing
These analyses can provide insights into the expression patterns of different genes in the neuronal cells, aiding the study of the molecular spectrum changes within the neuronal spheroids.	Placing neuronal spheroids on MEA allows real-time monitoring of neuronal electrical activity, studying the electrical signal transmission and network connectivity between neurons within the spheroids.

Data Analysis

Perform quantitative analysis of all obtained data, evaluate the spheroids' response to all compounds or drugs, and rank them based on their toxicity levels.

Creative Biolabs Service Advantages

Professional Expertise and Experience
State-of-the-Art Equipment and Automation Technology
Customized Solutions and Flexibility
Delivery of High-Quality Accredited Assessment Results

Creative Biolabs will be your preferred partner because our services based on the 3D neuronal spheroid model are undeniably an attractive and reliable choice. Additionally, we offer other experimental models that closely resemble human physiological conditions:

3D Microtissue Model

Ex Vivo Human Peripheral Sensory Neurons

Brain Organoid

For clients seeking high-quality neurotoxicity evaluation services or other related experimental models, we sincerely encourage you to contact us. We are dedicated to providing excellent support and solutions for your research and projects.

Reference

Wang, Qin et al. "Assessment of a 3D neural spheroid model to detect pharmaceutical-induced neurotoxicity." ALTEX. 39,4 (2022): 560-582. Distribution under Open Access license CC BY 4.0, without modification.

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

Related Sections:

Research Models

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