This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-mPGK-Sec/SPI-CD79α-CD28-HA) and CRISPR-Cas (Cas12b (C2c1)-dsDNA-HD/Cas3-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | TKA3383 |
Host | Rabbit |
Targeting Cell Type | CA46 |
Reporter gene | RFP |
Promoter | mPGK |
Signal peptides | Sec/SPI |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | HA |
Linkers | Flexible Linker |
Co-Receptors | FcγRIIB |
Vector Type | Lentivirus |
Vector Name | BGpCDH2 |
Class | 1 multi-Cas |
Type | I |
Subtype | A |
Effector | Cas12b (C2c1) |
DNA Type | dsDNA |
Nuclease domains | HD/Cas3 |
TracrRNA requirement | No |
PAM/PFS | NNGRRT |
Insertion | Wap |
Construction | RFP-mPGK-CD79α-Cas12b (C2c1)-dsDNA-Wap |
Target | S100A1 |
Alternative Names | S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1 |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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