S100A1 mAb (TKA3383) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR25)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-mPGK-Sec/SPI-CD79α-CD28-HA) and CRISPR-Cas (Cas12b (C2c1)-dsDNA-HD/Cas3-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone TKA3383
Host Rabbit
Targeting Cell Type CA46
Reporter gene RFP
Promoter mPGK
Signal peptides Sec/SPI
Signaling domains CD79α
TM domains CD28
Detection tags HA
Linkers Flexible Linker
Co-Receptors FcγRIIB
Vector Type Lentivirus
Vector Name BGpCDH2
Class 1 multi-Cas
Type I
Subtype A
Effector Cas12b (C2c1)
DNA Type dsDNA
Nuclease domains HD/Cas3
TracrRNA requirement No
PAM/PFS NNGRRT
Insertion Wap
Construction RFP-mPGK-CD79α-Cas12b (C2c1)-dsDNA-Wap

Target Information

Target S100A1
Alternative Names S100A1; S100; S100-alpha; S100A; S100 calcium-binding protein A1; S100 calcium binding protein A1

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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