4-[3-(Trifluoromethyl)-3H-diazirin-3-yl]benzoic Acid (TDBA) Photo-reactive Crosslinker (CAT#: OLY-1023-YS33)

Description

TDBA is a heterobifunctional, photo-reactive crosslinker used for photoaffinity labeling (PAL). It possesses a carboxylic acid group, which can be activated (e.g., with EDC/NHS) to couple with primary amines on a 'bait' molecule. The other end features a trifluoromethyl-diazirine group. This moiety is stable in the dark but, upon activation with UV light (approx. 350-370 nm), generates a highly reactive carbene. This carbene non-selectively inserts into nearby C-H, N-H, or O-H bonds of an interacting 'prey' protein, permanently capturing transient interactions and enabling target identification.

Molecular Weight

230.15

Purity

>98.0%

Formula

C₉H₅F₃N₂O₂

Product Type

Heterobifunctional Crosslinkers ; Photo-reactive Crosslinkers

Group 1

CHO

Group 2

X (F, Cl, Br, I)

Form (20 deg.C)

Solid

Storage Condition

Store under inert gas, <0°C

Molecular Structure