Disuccinimidyl Suberate (DSS) Homobifunctional Crosslinker (CAT#: OLY-1023-YS132)

Description

DSS is a classic homobifunctional, amine-reactive crosslinker. It features two N-hydroxysuccinimide (NHS) ester groups separated by an 8-carbon (C8) aliphatic suberate spacer. NHS esters react efficiently with primary amines (lysines) at pH 7.0-9.0 to form stable, non-cleavable amide bonds. DSS is widely used to study protein-protein interactions by covalently "freezing" them. Its medium-length, hydrophobic spacer arm makes it membrane-permeable, allowing for crosslinking of proteins both in vitro and inside living cells. It is the non-cleavable analogue of DSP.

Molecular Weight

368.34

Purity

>98.0%

Formula

C₁₆H₂₀N₂O₈

Product Type

Homobifunctional Crosslinkers

Group 1

NHS

Group 2

NHS

Form (20 deg.C)

Solid

Storage Condition

Store under inert gas, 2-8°C

Molecular Structure