Custom Oligonucleotide Synthesis & Modification Service
Unlock the Full Potential of Your Research! Are you facing challenges with oligonucleotide stability, delivery, or specificity in your therapeutic and diagnostic development? Our Custom Modified Oligos Synthesis & Modification Service helps you overcome these hurdles to accelerate your research. Through advanced chemical modification, precision bioconjugation, and rigorous quality control, we deliver high-performance oligonucleotides engineered for superior stability, enhanced cellular uptake, and precise targeting, ensuring the success of your most critical applications.
Introduction
Oligonucleotides are at the forefront of biomedical innovation, serving as the core components for antisense therapies, siRNAs, and high-specificity diagnostic probes. However, standard oligonucleotides often suffer from rapid degradation and poor cellular uptake. Strategic chemical modification is essential to enhance their therapeutic and diagnostic efficacy. By incorporating modifications like phosphorothioates or 2'-O-Methyl groups, we can significantly improve nuclease resistance, binding affinity, and overall performance, transforming a promising sequence into a powerful research tool or therapeutic candidate.
Fig.1 Construction and application of oligonucleotide probes.
How Can We Assist Your Project?
At Creative Biolabs, we translate your sequence into a functionally optimized molecule ready for demanding applications. We specialize in synthesizing highly modified and complex oligonucleotides that standard providers cannot produce. Whether you need to enhance the stability of an antisense therapeutic, increase the specificity of a diagnostic probe, or conjugate your oligo to a targeting ligand for precision delivery, our expert team provides end-to-end support. We deliver publication-quality, functionally validated oligonucleotides tailored to your exact specifications, empowering you to achieve reliable, reproducible results and accelerate your project timelines.
Types of Our Service
Specialized Oligo Constructs
We synthesize a variety of specialized and ready-to-use oligo formats to support cutting-edge research areas.
- Plain DNA/RNA
- Antisense Oligonucleotides (ASOs)
- Small interfering RNA (siRNA)
- Aptamers
Custom Chemical Modifications
Bioconjugation Services
Workflow: From Sequence to Solution
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Required Starting Materials: To begin, we typically require key project details such as:
- The complete DNA or RNA sequence.
- A list of desired modifications (e.g., 2'-O-Methyl, Phosphorothioate linkages, LNA bases, 5' Biotin).
- The intended application (e.g., antisense, siRNA, qPCR probe, in vivo study) helps our experts recommend the optimal design and purification strategy.
- Key Steps Involved:
| Step | Description |
|---|---|
| Consultation & Strategic Design | Our PhD-level scientists consult with you to understand your goals, review your sequence, and finalize the modification strategy to maximize performance and manufacturability. |
| High-Fidelity Chemical Synthesis | Your oligonucleotide is synthesized on our state-of-the-art platform with real-time monitoring to ensure exceptional coupling efficiency (>99%). |
| Precision Modification & Conjugation | Requested modifications are incorporated during or after synthesis. For conjugations (e.g., to peptides, antibodies, or dyes), we employ optimized chemical ligation strategies to ensure high yield and preserve molecular function. |
| Rigorous Purification | The crude product undergoes a stringent purification process, typically multi-step HPLC, to remove truncated sequences and other impurities, achieving the high purity level required for your application. |
| Comprehensive Quality Control & Formulation | Every oligo is subjected to rigorous QC analysis, including mass spectrometry (to verify identity and molecular weight) and analytical HPLC (to confirm purity). The final product is then formulated (e.g., lyophilized or in a specific buffer) according to your needs. |
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Final Deliverables: Upon completion, you will receive a comprehensive package including:
- The final, purified oligonucleotide at the specified quantity.
- A detailed Certificate of Analysis (CoA) with all QC data, including mass spectrometry and HPLC traces.
- Estimated Timeframe: The typical timeframe for this service ranges from 2 to 5 weeks. Project duration is influenced by the complexity of the sequence, the number and type of modifications, and the scale of the synthesis.
Why Choose Us?
Choosing Creative Biolabs means partnering with a team dedicated to scientific excellence and client success. We thrive on complex challenges that other suppliers turn away. Our key advantages include our proprietary synthesis protocols for sensitive modifications, an extensive portfolio of chemical modifications, and an unwavering commitment to quality. We don't just deliver a product; we provide a reliable, high-performance solution backed by expert support.
Customer Reviews
"Using Creative Biolabs' Custom Modified Oligos Synthesis & Modification Service in our research has significantly improved our antisense program. The purity of their phosphorothioate-modified oligos is exceptional, which has drastically reduced the toxicity we observed with products from other vendors. The comprehensive CoA gives us full confidence for our animal studies."
— Dr. Em***a Chen [June 2025]
"Attaching our custom peptide to an LNA-gapmer was a major challenge until we found Creative Biolabs. Their team handled the entire workflow seamlessly, from oligo synthesis to conjugation and purification. Using this construct has significantly improved the cellular uptake and efficacy in our targeted therapy research."
— Dr. Sa***l Jones [May 2025]
"The batch-to-batch consistency of the dual-labeled probes from Creative Biolabs has been a game-changer for our diagnostic assay development. Their technical support is also top-notch."
— Dr. An**a Patel [November 2024]
FAQs
Can you synthesize long or highly complex sequences with multiple modifications?
A: Yes, this is our specialty. Our advanced synthesis and purification platforms are optimized for producing long oligonucleotides (up to 200 bases) and incorporating multiple complex modifications, including chimeric backbones and various conjugated ligands. We encourage you to send us your most challenging sequences for a feasibility assessment.
What quality control measures do you have in place?
A: Every oligonucleotide undergoes rigorous quality control. We verify the identity and molecular weight of every oligo by mass spectrometry (LC-MS) and confirm its purity using analytical HPLC. All data is provided to you in a comprehensive Certificate of Analysis, ensuring full transparency and traceability.
What is the typical turnaround time for a custom modified oligo?
A: Turnaround time depends on the complexity of your project, but typically ranges from 2 to 5 weeks. Simple modifications may be completed faster, while multi-step conjugations or very long sequences may require more time. We will provide a specific estimated timeline with your formal quote.
How do you handle sequences that are known to be difficult to synthesize, such as those with high GC content or repetitive regions?
A: Our scientific team has extensive experience with difficult sequences. We employ specialized synthesis protocols, including optimized reagents and cycle conditions, to improve coupling efficiency and minimize failure sequences for GC-rich or repetitive templates, ensuring the highest possible yield and purity.
I need a modification that isn't listed on your website. Can you still help?
A: Most likely, yes. We have a vast network of raw material suppliers and extensive chemistry expertise. If a modification is chemically feasible, we can likely incorporate it into your oligonucleotide. Please contact our team to discuss your specific needs.
Related Services
To fully support your research from gene to function, Creative Biolabs offers a suite of complementary services that integrate seamlessly with our oligonucleotide synthesis.
Custom Peptide Synthesis
Synthesize high-purity peptides for use as cell-penetrating carriers or targeting ligands in your oligonucleotide conjugation projects.
Recombinant Protein Expression
Express and purify the protein target of your antisense or siRNA oligonucleotide to validate its functional effect in vitro.
Antibody Production Services
Develop custom antibodies for creating highly specific antibody-oligonucleotide conjugates (AOCs) for targeted therapeutic delivery.
How to Contact Us?
Creative Biolabs is your dedicated partner for advancing oligonucleotide-based research and therapeutics. Our commitment to quality, scientific expertise, and customer collaboration ensures you receive functionally superior molecules tailored for success. Contact our team for more information. Our experts are ready to provide a detailed consultation, assess the feasibility of your design, and provide a formal quote to get your project started.