What types of assays do you use for apoptosis screening?

We offer a wide range of assays, including but not limited to fluorescence-based binding assays, cell viability assays, caspase activation assays, and high-content imaging. The choice of assay is customized to your specific target and research goals.

How do you guarantee the quality of your screening results?

We employ strict quality control measures throughout our workflow, including the use of optimized, validated protocols, positive and negative controls in every assay, and state-of-the-art instrumentation. Our expert team also performs rigorous data analysis and interpretation to ensure the highest reliability.

Is your service compatible with different types of compounds?

Yes, our platform is highly versatile and compatible with a wide range of compounds, including small molecules, peptides, and natural product extracts. We can adapt our protocols to accommodate the specific properties of your compound library.

Can your service help me determine the specific pathway my compound affects?

Absolutely. Our profiling service is specifically designed to elucidate the mechanism of action. By analyzing the activation of key proteins (e.g., caspases) and other downstream events, we can provide a detailed picture of how your compound modulates apoptosis.

Do you offer services for both pro-apoptotic and anti-apoptotic drug discovery?

Yes, our services are designed to support both pro-apoptotic strategies for oncology and anti-apoptotic strategies for conditions such as neurodegenerative diseases and ischemic injury. We can customize the assays to screen for both types of modulators.

Apoptosis Screening & Profiling Service

Overview

Apoptosis, a regulated form of cell death, is a fundamental mechanism observed in multicellular organisms. Apoptosis plays an important role in many biological processes, such as immune system homeostasis, and hormone-driven atrophy. Comprehending the underlying mechanism of apoptosis holds significant importance, as it assumes a central role in the therapeutic management of cancer and various other ailments. Consequently, the screening and profiling of apoptosis-related pathways has profound significance for the development of drugs targeting apoptosis-related proteins.

Fig.1 Apoptosis pathways.1 Fig. 1 Apoptosis pathways. (Chaudhry, et al., 2022)

Our Apoptosis Screening & Profiling Service

With years of experience and expertise in apoptosis signal pathways, Creative Biolabs succeeds in providing global customers with comprehensive apoptosis screening & profiling services. We have several detection methods to perform apoptosis screening & profiling, one of which is by measuring the fluorescence signal after incubation with an apoptosis target bound to its ligand. Meanwhile, we offer a variety of apoptosis targets-related services to empower customers' drug discovery programs. The amalgamation of our specialist team and extensive range of services ensures that we deliver high-quality data with expeditious turnaround time.

Fig.2 The bioluminescent annexin assay concept. 2 Fig. 2 The bioluminescent annexin assay concept. (Kupcho, et al., 2019)

Our Service Workflow: From Concept to Clinical

Target and Assay Setup: We work with you to select the appropriate target and design a custom high-throughput screening (HTS) assay based on your specific requirements. We utilize methods such as fluorescence-based assays to measure protein-protein interactions or cell viability.
Primary High-Throughput Screening: We perform a rapid, large-scale screening of your compound library using our automated platform to identify potential hits that modulate the target pathway.
Secondary Validation and Dose-Response Studies: Hits from the primary screen are subjected to secondary assays to confirm their activity and determine their dose-response relationships (e.g., IC50 values).
Mechanism of Action Analysis: We perform detailed profiling studies to determine the precise mechanism by which your compounds induce or inhibit apoptosis, including identifying key proteins and downstream signaling events.
Data Analysis and Reporting: Our expert scientists analyze all the data and compile it into a comprehensive report, including raw data, interpreted results, and detailed recommendations for the next steps in your drug discovery project.

Required Starting Materials

Target proteins or cells of interest (e.g., Bcl-2 family proteins, caspases, cancer cell lines).
A list of small molecule compounds or a library to be screened.
Detailed project goals, including desired readouts or specific apoptosis pathways to be investigated.

The Breadth of Our Apoptosis Screening & Profiling Service

Fig.5 Fig. 3 The breadth of our apoptosis screening & profiling service. (Creative Biolabs Original)

Customer Reviews

[High-Quality Leads] Dr. A. L****y, Director of Drug Discovery.

Using Creative Biolabs' service in our research has significantly improved the quality of our lead compounds. The detailed profiling data allowed us to prioritize candidates with the most promising mechanisms of action, saving us significant time and resources.

[Efficient Validation] R. P***o, Senior Scientist.

The comprehensive validation and dose-response studies provided by Creative Biolabs were essential for our project. The data were clear, precise, and directly supported our therapeutic hypothesis, enabling us to move to in vivo studies with confidence.

[Targeted Approach] Dr. K. J****s, Principal Investigator.

We faced challenges with our in-house screening due to the complexity of the apoptosis pathway. Creative Biolabs' team expertly designed a custom assay that specifically addressed our research question, providing us with invaluable data that we couldn't obtain elsewhere.

FAQs

Q1: What types of assays do you use for apoptosis screening?

A1: We offer a wide range of assays, including but not limited to fluorescence-based binding assays, cell viability assays, caspase activation assays, and high-content imaging. The choice of assay is customized to your specific target and research goals.
Q2: How do you guarantee the quality of your screening results?

A2: We employ strict quality control measures throughout our workflow, including the use of optimized, validated protocols, positive and negative controls in every assay, and state-of-the-art instrumentation. Our expert team also performs rigorous data analysis and interpretation to ensure the highest reliability.
Q3: Is your service compatible with different types of compounds?

A3: Yes, our platform is highly versatile and compatible with a wide range of compounds, including small molecules, peptides, and natural product extracts. We can adapt our protocols to accommodate the specific properties of your compound library.
Q4: Can your service help me determine the specific pathway my compound affects?

A4: Absolutely. Our profiling service is specifically designed to elucidate the mechanism of action. By analyzing the activation of key proteins (e.g., caspases) and other downstream events, we can provide a detailed picture of how your compound modulates apoptosis.
Q5: Do you offer services for both pro-apoptotic and anti-apoptotic drug discovery?

A5: Yes, our services are designed to support both pro-apoptotic strategies for oncology and anti-apoptotic strategies for conditions such as neurodegenerative diseases and ischemic injury. We can customize the assays to screen for both types of modulators.

Work with Creative Biolabs

Fig.4 Contact Flowchart.

Creative Biolabs provides best-in-class service that allows for full customization and design from pilot studies to special targeted projects, meaning the service is specifically tailored to answer customers' biological questions. Our competent team, together with our diverse service portfolio, gives us confidence in providing high-quality data in a timely manner.

For more details on our apoptosis screening & profiling service, please feel free to contact us. We are pleased to work together with global customers' attractive projects.

References

Chaudhry, Gul-e-Saba, et al. "Cancer and apoptosis: The apoptotic activity of plant and marine natural products and their potential as targeted cancer therapeutics." Frontiers in Pharmacology 13 (2022): 842376.
Distributed under Open Access License CC BY 4.0, without modification.
Kupcho, Kevin, et al. "A real-time, bioluminescent annexin V assay for the assessment of apoptosis." Apoptosis 24 (2019): 184-197.
Distributed under Open Access License CC BY 4.0, without modification.

For Research Use Only.

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