Apoptosis is a tightly controlled process of programmed cell death that serves as a mechanism for eliminating damaged or aging cells. There are several drugs have been discovered targeting tumor cell apoptosis. Therefore, apoptosis assay serves as a crucial type of detection assay for cancer drug discovery and development. Developing robust apoptosis assays will help global clients to speed up the development of drug candidates.
Fig.1 The 'apoptosis paradox' in cancer.1
Creative Biolabs widely provides a cost-effective PathSpecific™ apoptosis assay service for global customers to support your drug discovery targeting apoptosis. In this service, we examine the absorbance and fluorescence as the indicator in response to test sample treatment utilizing several well-developed approaches to enable our PathSpecific™ apoptosis assay service. In addition, we offer a select number of apoptosis targets involved in different apoptosis pathways to aid in our PathSpecific™ apoptosis assay service and also deliver related assays for your convenience. Moreover, we offer a comprehensive customized service to guarantee ideal results for every customer at Creative Biolabs in a rapid timeline.
| Approaches | Readout | |
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Molecular interaction measurement in a consistent format is achieved by integrating time-resolved measurement (TR) with fluorescence resonance energy transfer (FRET). | Fluorescence |
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The principle of the fluorescence polarization experiment is that the degree of excitation of small peptides with fluorescent labels by polarized light varies with the rotation angle. | |
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Quickly distinguishing apoptosis from necrosis, accurately counting apoptosis cells, and determining whether apoptosis occurs in its early or late phases are all possible using flow cytometry detection assay. | |
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The MTT test is a colorimetric method to evaluate mitochondrial activity, which is an indicator of cellular viability. | Absorbance |
Here we list several apoptosis targets and provide related assays to aid in our PathSpecific™ apoptosis assay service.
| Cat | Targets | Related Assays |
| ZL-1122-LX137 | BCL-2 | PathSpecific™ BCL2 Apoptosis Assay |
| ZL-1122-LX138 | BCL-xL | PathSpecific™ BCL-xL Apoptosis Assay |
| ZL-1122-LX139 | cIAP-1 | PathSpecific™ cIAP-1 Apoptosis Assay |
| ZL-1122-LX140 | cIAP-2 | PathSpecific™ cIAP-2 Apoptosis Assay |
| ZL-1122-LX69 | Caspase-1 | PathSpecific™ Caspase 1 Protease Assay |
| ZL-1122-LX73 | Caspase-2 | PathSpecific™ Caspase 2Protease Assay |
| ZL-1122-LX74 | Caspase-3 | PathSpecific™ Caspase 3 Protease Assay |
| ZL-1122-LX75 | Caspase-4 | PathSpecific™ Caspase 4 Protease Assay |
| ZL-1122-LX76 | Caspase-5 | PathSpecific™ Caspase 5 Protease Assay |
| ZL-1122-LX77 | Caspase-6 | PathSpecific™ Caspase 6 Protease Assay |
| ZL-1122-LX78 | Caspase-7 | PathSpecific™ Caspase 7 Protease Assay |
| ZL-1122-LX79 | Caspase-8 | PathSpecific™ Caspase 8 Protease Assay |
| ZL-1122-LX80 | Caspase-9 | PathSpecific™ Caspase 9 Protease Assay |
| ZL-1122-LX70 | Caspase-10 | PathSpecific™ Caspase 10 Protease Assay |
| ZL-1122-LX71 | Caspase-11 | PathSpecific™ Caspase 11 Protease Assay |
| ZL-1122-LX72 | Caspase-14 | PathSpecific™ Caspase 14 Protease Assay |
| ZL-1122-LX68 | Calpain 1 | PathSpecific™ Calpain 1 Protease Assay |
Representative data: This study focuses on understanding the apoptotic response of eukaryotic cells under ER stress. The obtained data shows that increased levels of GRP78 can suppress apoptosis induced by ER stress, thus reducing the overall amount of cell death during chondrogenesis triggered by BMP2. These findings shed light on the essential function of GRP78 in managing ER stress-related apoptosis in cartilage formation and molecular processes.
Fig.2 FCM-based cellular apoptosis assays.2
If you want to know more details about our PathSpecific™ apoptosis assay service, please don't hesitate to contact us at your convenience.
References
For Research Use Only.
