Antibodies have emerged as key players in modern medical treatments, exploiting the body's immune system for targeted therapy. Their exceptional precision and strength in binding to specific antigens enable highly effective treatments with minimal side effects. Additionally, the flexibility in their structure and functions allows for a diverse range of therapeutic applications and targets. With the advancement of engineering technologies, antibodies can be modified for improved efficacy, underscoring the importance of thoroughly understanding their functions and therapeutic potential for advancing immunotherapy.
Fig.1 The immune responses induced by therapeutic monoclonal antibodies in cancer treatment.1, 4
Therapeutic antibodies leverage diverse mechanisms of action like receptor blocking, receptor activation, and immune cell-mediated killing processes for targeting diseases. This necessitates a varied approach to evaluate these functions and enhance antibody performance through engineering methods.
At Creative Biolabs, we take pride in offering an extensive range of assays designed to assess the functionality of antibody-based therapeutics. Our assays focus on key antibody mechanisms, including Fab-based antigen-antibody binding and Fc-based antibody function. Leveraging our advanced research platforms and experienced team, we are well-equipped to assist customers worldwide in developing and executing optimal project schemes with high effectiveness and reliability. We are committed to providing proven project results that enable thorough characterization of antibody function and facilitate the selection of promising candidates during the discovery phase.
Fig.2 Representation of factors influencing antibody targeting in the biological environment.2, 5
We utilize a variety of assay formats and technical methods to properly understand the characteristics of the variable domain, including:
| Services | Overview | Services | Overview |
|---|---|---|---|
| Antibody Binding Assays | Combined with numerous state-of-the-art techniques, we globally provide multiple assay formats such as cell- or non-cell-based binding assays, antibody cross-reactivity examing, etc., to advance antibody drug discovery and development. | In Vitro Cellular Senescence Assay | Unlock the secrets of cellular aging with our cutting-edge cellular senescence assay, which utilizes β-gal activity detection methods to provide valuable insights. |
| Cellular Proliferation Assays | Our proliferation assays provide valuable insights into the cytotoxic effects of antibodies, aiding in assessing therapeutic consequences and pharmacological safety. | PARP Assay | Our PARP assay services are designed to support the discovery of PARP-targeted therapeutics for cancer treatment. We offer comprehensive testing to aid cancer research efforts. |
| Cellular Apoptosis Assays | Our high-throughput caspase screening assays provide valuable insights into the mechanisms of cell death mediated by antibodies, helping to identify specific pathways involved in apoptosis and better understand their impact on target cells. | In Situ MicroRNA Detection Service | We utilize fluorescence in situ hybridization (FISH) to analyze miRNA expression in situ |
| Neutralization Assays | Our neutralization assays provide a comprehensive platform to evaluate and quantify the neutralization potential of antibodies against a range of pathogens, cytokines, toxins, and other targets, allowing for tailored assay formats to suit your specific research needs. | In Vitro Cell-based α-synuclein Aggregation Assay Service | We leverage apoptosis induction to stimulate the formation of α-synuclein aggregates, enabling the measurement of α-synuclein expression and aggregation levels, which can inform the development of therapeutic strategies. |
| Antibody-Receptor Function Studies | In our antibody-receptor functional analysis services, we evaluate interactions with immune checkpoints and G protein-coupled receptors (GPCRs) to improve immunotherapy strategies. | In Vitro Cellular Phosphorylation Assay Service | Our assay efficiently measures phosphorylation levels by leveraging endogenously expressed target kinases in cells or utilizing cells engineered for overexpression of the specific kinase under study. |
| Endocytosis and Internalization Assays | The internalization process is important for maintaining cell function and can be studied for understanding diseases and developing new therapies. We offer cutting-edge techniques to investigate and manipulate internalization processes for research purposes | In Vitro Cell-based Phospholipidosis Assay Service | We offer a cell-based assay that utilizes a fluorescent dye to quantify PLD changes in cells. By observing fluorescence intensity changes, we can rapidly examine compounds for their potential to induce phospholipidosis. |
| Angiogenesis Investigation | Our angiogenesis investigation services pay the way for tumor progression and metastasis research involving tube formation, cell migration, cell adhesion, etc. |
Fig.3 Antibody Fc-mediated immunomodulation.3, 5
Creative Biolabs specializes in evaluating and optimizing Fc regions, including glycosylation, to improve safety and efficacy. Our advanced technology platforms enable tailored engineering strategies for optimizing antibody function. Our services include:
| Services | Overview |
|---|---|
| Fc-mediated Effector Functions | We specialize in analyzing and interpreting the cytotoxic effects of candidates using ADCC, CDC, and ADCP assays. Additionally, we offer non-cell-based assays to characterize antibody products with Fcγ receptor binding capabilities and assess binding affinity to complement component C1q. |
| Fc Engineering | Creative Biolabs provides services for Fc engineering to fine-tune antibody properties and enhance efficacy. This includes antibody skeleton engineering and Fc glycoengineering, optimizing effector functions for improved therapeutic outcomes. |
Q1: What sets a binding assay apart from a functional assay?
A1: It is important to distinguish between the functional activity and binding activity of antibodies. While binding activity refers to the ability of an antibody to bind to its target antigen, functional activity pertains to the biological effects it induces, such as cell signaling or immune response modulation. Functional assays are designed to assess the specific biological impact of an antibody, providing crucial insights into its therapeutic potential.
Q2: What are the requirements for antibody function assays?
A2: To conduct antibody functional assays, we need customers to provide basic information about your candidate samples, such as name, molecular weight, etc. Different assays have different sample submission requirements. We provide one-on-one consulting services to ensure that your project goes smoothly. Please reach out to our team if you require any specific accommodations or assistance.
Want to know more about your antibodies'? Please contact us with your meaningful projects.
References
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