In Vitro Cell Adhesion Assay

Creative Biolabs specializes in providing in vitro cell adhesion assay services to support our customers' scientific research in global cancer and related biomedical industries.

Overview of Adhesion Assay

Adhesion of a cell to an extracellular matrix (ECM), or a certain surface is a complex process involving many different molecular interactions. It is critical for the cell's growth and survival as well as communication with other cells. Establishing an appropriate in vitro cell co-culture system to detect the response of tumor cells adhering to stromal cells or extracellular matrix is of great significance for discovering new drugs for treating cancer pathology.

Fig.1 Dynamic cell adhesion cascade with docking and locking phases. (Khalili, 2015)

Our In Vitro Cell Adhesion Assay Service

Creative Biolabs specializes in providing in vitro cell adhesion assay services allowing global customers to test the response of a specific type of cell or cell line to adhere to a specific adhesive substrate, as well as the regulatory effects of the drug on unique cell-substrate interaction.

In our in vitro cell adhesion assay, cells are labeled with fluorescent probes and added to stromal cell monolayers or extracellular matrix-fixed plates for experiments. And a quantitative method based on a fluorescence measurement system was used to determine the number of adherent cells. Throughout the process, factors such as cell type, extracellular matrix substrate, test product concentration, and treatment time all have an impact on the final results. Creative Biolabs provide optimization of the whole experiment according to the customer's project.

Fig.2 Schematic of cell adhesion assay. (Weitz-Schmidt, 2011)

Service Workflow

We provide comprehensive in vitro adhesion assay services with a range of process operations from cell culture, test articles treatment, and data analysis. We also provide customized service optimization according to the particularity of customer projects.

Fig.3 Flowchart of in vitro adhesion assay. (Creative Biolabs)

Our Features & Benefits

Published Data

Here are some results displayed that refer to the drawings of cell adhesion assay articles.

1. Endothelial cell alterations induced by inflammatory smooth muscle cells affect cerebral aneurysm progression by regulating the expression of integrin and VEGF.

Fig.4 Cell adhesion assay of HUVECs treated with collagen subtypes. (Liu, 2019)

2. PLCε1 promotes SDF-1α–induced lymphocyte adhesion and migration to the inflammation sites.

Fig.5 SDF-1α–induced morphological changes are dependent on PLCε1. (Strazza, 2017)

For more details about our in vitro cell adhesion assay, please feel free to contact us or directly sent us an inquiry.

References

1. Khalili, A.A.; et al. A Review of Cell Adhesion Studies for Biomedical and Biological Applications. Int. J. Mol. Sci. 2015, 16, 18149-18184.
2. Weitz-Schmidt, G.; et al. Cell adhesion assays. Methods Mol Biol. 2012; 757:15-30.
3. Dutta, A.; et al. Culture of K562 human myeloid leukemia cells in presence of fibronectin expresses and secretes MMP-9 in serum-free culture medium. Int J Clin Exp Pathol. 2010, 3(3):288-302.
4. Liu, P.; et al. Inflammatory Smooth Muscle Cells Induce Endothelial Cell Alterations to Influence Cerebral Aneurysm Progression via Regulation of Integrin and VEGF Expression. Cell Transplant. 2019, 28(6):713-722.
5. Strazza, M.; et al. PLCε1 regulates SDF-1α-induced lymphocyte adhesion and migration to sites of inflammation. Proc Natl Acad Sci U S A. 2017, 114(10):2693-2698.

For Research Use Only.