Rodent models have long been used as tools to interrogate both the pathophysiology and sequelae of breathing-related abnormalities during sleep and to improve approaches to diagnosis and therapy. Especially, Creative Biolabs offers intermittent hypoxia exposure rodent models of sleep apnea for clients that are interested in related areas.
Sleeping-disordered breathing refers to intermittent, somewhat cyclical reduction or cessation of airflow during sleep. In the specific disorder known as sleep apnea, airflow stops during sleep. It is divided into two subtypes, obstructive sleep apnea (OSA) and central sleep apnea. OSA is more prevalent and is characterized by repetitive occlusions of the upper airway during sleep resulting in intermittent hypoxia (IH), increased respiratory efforts, and sleep fragmentation due to a respiratory effort aimed at opening the collapsed airway. It can lead to various comorbid conditions, including diabetes and cardiovascular disease. Moreover, IH and sleep disruption can negatively affect insulin sensitivity and glucose regulation, contributing to the development of metabolic syndrome. Additionally, OSA can cause neurocognitive effects, such as daytime sleepiness, impaired memory and concentration, and persistent cognitive impairment. Further, IH has been reported to be associated with tumor growth increase and metastasis.
Fig.1 Obesity predisposes to obstructive sleep apnoea syndrome (OSAS). (Levy et al. 2015)
Many experimental models have been developed to improve the understanding of the pathophysiological link between OSA and other diseases, as well as to evaluate the efficacy of potential therapeutics. The intermittent hypoxia model exposes animals to repetitive hypoxia and oxygenation. Animals are placed in a ventilated cage or chamber, where the can breathe nitrogen-enriched air (to create hypoxia) alternating with oxygen or normal air. The cycle can be repeated every day for several weeks. Moreover, an advantage of this model is that they permit exposures that can be extended over months, allowing the investigation of chronic consequences that might occur in humans.
Creative Biolabs offers IH exposure-induced rodent models of OSA to study the effect of drug candidates on this disease and approach its possible mechanism. The vital signs of animals can be monitored every day and blood gas analysis can be performed by taking suction arterial blood. The sleeping state can be monitored with polysomnography [electroencephalogram (EEG), electromyogram (EMG) and electrooculogram (EOG)]. The apnea time can be recorded. Besides, we can offer many other assessments including but not limited to:
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At Creative Biolabs, you will find the most comprehensive neurological preclinical drug efficacy testing services. To better serve the unique requirements of our clients, we can offer customized models and protocols, or validate existing models from the literature. The list for the broad spectrum of our rodent disease model services is as follows:
Creative Biolabs helps our clients to choose the most suitable models based on your research purpose and requirements. Our seasoned staff will give updates of project progression regularly to keep you how far along progress is. Moreover, our well-established service process ensures the accomplishment of projects in a timely and efficient manner. If you are interested in our services, please contact us to discuss your research needs.
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