The detrimental effects of insomnia/sleep loss have been reported a lot. In order to unveil the sleep function, study the effects of sleep loss on brain functions, and evaluate drug effect on brain function repair, experimental procedures of sleep deprivation (SD) have been developed. Especially, Creative Biolabs offers both partial and total SD rat and mice models for clients globally to test the ability of drugs to reverse SD-induced cognitive impairments and memory loss.
Sleep is critical for proper development and neural plasticity. Abnormal sleep patterns and behavioral deficits are characteristics of many neurodevelopmental disorders, including autism spectrum disorders, tuberous sclerosis complex, and fragile x syndrome. Given the number of neurodevelopmental disorders that are associated with abnormal sleep patterns, it is critical to establish animal models to understand how lack of sleep affects brain function and behavior, and to assess the efficacy of drug candidates to improve sleep loss-induced brain dysfunctions at the molecular, cellular, physiological, and cognitive levels.
Different distinct methodologies that restrict or deprive animals of sleep have been developed, such as environmental changes, discomfort, pharmacological induction, genetic models, and physical induction. Physical induction, such as forced locomotion technique and platform method, are more widely used models of SD. Based on different research purposes, Creative Biolabs offers different types of SD models in mice and rats, including total SD and partial SD.
Fig.1 Sleep loss and hippocampal memory encoding. (Levy et al. 2015)
Sleep in mammals includes two states: rapid eye movement (REM) sleep and non-REM (NREM) sleep. They alternate in cycles, with NREM sleep preceding REM sleep epochs. Total sleep deprivation refers to complete removal of sleep for a desired amount of time, independent of sleep state. In partial or state-dependent SD, on the other hand, a specific sleep state is selectively targeted for deprivation. Mostly, REM sleep is targeted but the select of sleep state depends on the particular function under study or the particular deficit to be elicited. At Creative Biolabs, we offer both choices for our clients which can be customized to respond to customer requirements.
Creative Biolabs offers a wide range of assessments to assess the effect of drugs on sleep and brain functions. Sleep state monitoring is performed with the aid of detection of electrophysiological signs, including electroencephalogram (EEG), electromyogram (EMG) and electrooculogram (EOG). Moreover, as SD can lead to memory impairment in rodents, we offer different cognitive function analysis to investigate the effect of drugs to reverse SD-induced memory impairments. In short, we offer assessments including but not limited to:
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Creative Biolabs provides a comprehensive list of rodent nervous system models for your drug discovery projects. You might also be interested in the following areas:
With a scientific technical team experienced with model establishment and validation, Creative Biolabs can offer high-quality cooperative model development services at reasonable prices. Moreover, we can provide full-service packages that integrate in vivo pharmacology services with pharmacokinetic (PK) studies, in vitro ADME studies, and ex vivo pharmacology studies. If you are interested in our services, please contact us to discuss your research needs and get a formal quotation.
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