Adenovirus Vector

Adenoviral vectors have emerged as powerful tools in gene therapy research, offering exceptional advantages including high transduction efficiency, large packaging capacity, and robust gene expression. These versatile vectors provide remarkable flexibility in genetic modification while maintaining impressive safety profiles and manufacturing scalability. As the field continues to advance, researchers and developers seek reliable solutions to harness these capabilities effectively. At Creative Biolabs, we offer comprehensive adenoviral vector services spanning from sophisticated vector design and construction to scalable production and purification, empowering our partners with cutting-edge technology and deep expertise to accelerate their research and development goals. Our integrated platform combines innovative approaches with rigorous quality standards to deliver customized solutions that meet the evolving demands of gene therapy research.

UNDERSTANDING ADENOVIRAL VECTORS

Adenoviral vectors represent one of the most versatile and well-characterized gene delivery systems in molecular biology. While the structural and biological characteristics of adenoviruses provide the foundation for their utility (as shown in Table 1), their development into different vector generations has further expanded their applications and enhanced their safety profiles (detailed in Figure 1 and Table 2).

Table 1. Fundamental characteristics of adenoviruses

Characteristic	Description
Viral Structure	Double-stranded DNA virus (36 kb genome) Non-enveloped icosahedral capsid composed of hexon, penton and fiber proteins Diameter: ~70-90 nm Highly stable protein capsid structure
Serotypes	Over 50 human serotypes (Ad1-Ad57) Common serotypes: Ad5, Ad26, Ad35 Multiple animal serotypes available Different tissue tropisms based on fiber proteins
Genome Organization	ITR sequences for replication Early genes (E1-E4) for viral gene expression control Late genes (L1-L5) for structural proteins Packaging signal for viral assembly Multiple splicing regions
Cell Entry	Fiber-receptor interaction (CAR, CD46) Receptor-mediated endocytosis via integrins Efficient endosomal escape Active nuclear transport Rapid cellular processing
Expression Pattern	Strong natural viral promoters Efficient mRNA processing Robust protein production machinery Multiple regulatory elements Temporal gene expression control

Figure 1. Different generations of adenoviral vectors.(Creative Biolabs Original) Fig.1 Adenovirus vector construction for gene therapy

Table 2. Generations of adenoviral vectors: comparative analysis and applications

Vector Generation	Deletion/Modification	Packaging Capacity	Key Features	>Main Applications
First Generation	E1 region deletion (essential for viral replication) Optional E3 deletion (immune modulation) Retained viral genes: E2, E4 Most commonly used vectors	~8 kb	High transduction efficiency in multiple cell types Strong transgene expression (7-10 days) Relatively simple production process Well-established protocols Moderate immune response	Gene delivery for short-term expression Protein production and analysis Cancer gene therapy Vaccine development In vitro research applications
Second Generation	E1/E2/E3/E4 multiple gene deletions Reduced viral protein expression More complex complementing cell lines Enhanced safety features	~8 kb	Significantly reduced immunogenicity Extended expression duration (up to several weeks) Enhanced safety profile Improved transgene capacity Lower cellular immune response	Long-term gene therapy applications Chronic disease treatment Extended protein expression studies Advanced research applications Therapeutic gene delivery
Third Generation (HD-Ad)	All viral coding sequences deleted Only ITRs and packaging signal retained Required helper virus system Most advanced vector system	Up to 36 kb	Minimal immunogenicity and toxicity Long-term expression (months to years) Large transgene capacity Multiple gene delivery capability Highest safety profile	Complex genetic disorders Multi-gene delivery applications Large therapeutic gene transfer Long-term gene therapy Advanced vaccine development

OUR EXPERTISE & SERVICES

We provide end-to-end adenovirus vector development solutions with a focus on flexibility and precision to match your exact research specifications. Following our standard workflow, our customizable platform adapts to your unique project requirements while maintaining exceptional quality standards, enabling you to achieve optimal results through personalized approaches backed by our extensive expertise.

Initial Design Phase

Vector design strategy consultation
Selection of optimal adenovirus serotype
Transgene and promoter optimization
Vector capacity assessment
Regulatory element design
Safety consideration review

Recombinant Adenovirus Construction

Shuttle plasmid construction
Insertion of transgene expression cassette
Integration of regulatory elements
Verification of genetic elements
Quality control of intermediate constructs

Adenovirus Vector Production

Transfection and viral rescue
Initial viral stock generation
Viral amplification
Optimization of culture conditions
Scale-up strategies

Adenovirus Vector Purification

Crude lysate processing
Chromatography purification
Ultrafiltration/diafiltration
Final formulation
Concentration adjustment

Adenovirus Vector Characterization

Viral titer analysis (physical and infectious titers)
Comprehensive vector characterization (genome analysis, purity, stability)
Expression verification and potency assessment
Safety and purity testing (sterility, endotoxin, mycoplasma)
Complete documentation with detailed analysis reports and CoA

Delivery & Support

Optimized storage conditions and stability recommendations
Professional handling and shipping guidance
Comprehensive technical consultation and troubleshooting
Expert support for data interpretation and usage
Ongoing project assistance

Beyond our standard workflow, we offer specialized adenovirus vector development services to address diverse research needs and applications in vaccines and gene therapy. We also provide recombinant adenovirus vector products containing various promoters and shRNA-encoded genes. Explore our specialized services designed to meet your unique research requirements:

Adenovirus-Based cDNA Expression Libraries Generation Service

We construct comprehensive adenoviral cDNA expression libraries tailored to your research needs. Our service encompasses library design optimization, high-throughput cloning, quality-controlled vector production, and detailed characterization of the library complexity. We provide validated libraries with guaranteed coverage and diversity, supported by thorough documentation of library characteristics and screening protocols.

Helper-Dependent Adenoviral Vectors Service

We specialize in developing advanced helper-dependent adenoviral vectors with superior capacity and safety profiles. Our service covers the complete development process from vector design and helper virus system optimization to large-scale production and purification. We ensure high-quality HD-Ad vectors with minimal helper virus contamination, validated expression, and comprehensive quality testing.

Adenoviral Vector-based Vaccine Development

Our vaccine development service utilizes cutting-edge adenoviral vector technology to create effective vaccine candidates. We offer comprehensive solutions including antigen optimization, vector design, immunogenicity evaluation, and scalable production. Our platform supports both traditional and novel vaccination approaches, with extensive testing for safety, stability, and immune response.

Development of Adenoviral Vector as Immune Stimulant

We develop specialized adenoviral vectors designed to enhance immune responses. Our service includes vector modification for optimal immune stimulation, incorporation of immunomodulatory genes, and validation of immune activation. We provide detailed characterization of immune responses and support for mechanism-of-action studies.

Adenoviral Vector-based Suicide Gene Therapy Development

We offer complete development solutions for suicide gene therapy using optimized adenoviral vectors. Our service encompasses vector design with specific promoter systems, suicide gene optimization, safety feature integration, and validation of therapeutic efficacy. We ensure precise control of gene expression and thoroughly evaluate cell-killing efficiency.

Adenoviral Vector Design for RNAi Delivery

We design and develop specialized adenoviral vectors for efficient RNAi delivery. Our service includes custom shRNA/miRNA design, vector optimization for targeted delivery, expression validation, and knockdown efficiency assessment. We provide comprehensive solutions for both single and multiple RNAi target delivery systems.

Upstream Bioprocess Development for Adenovirus Vector

We optimize upstream processes for efficient adenoviral vector production. Our service covers cell line development, media optimization, culture parameter fine-tuning, and scale-up strategy development. We focus on maximizing vector yield while maintaining consistent quality through process validation and detailed documentation.

Downstream Process Development for Adenovirus Vectors

We develop robust downstream processes for adenoviral vector purification. Our service includes purification strategy design, chromatography method development, filtration optimization, and final formulation development. We ensure high purity, potency, and stability through comprehensive process validation and quality testing.

PLATFORMS & CAPABILITIES

Platform Capabilities of Adenovirus Vector Development Services. (Creative Biolabs Authorized)

Our state-of-the-art facilities are equipped with comprehensive analytical and production platforms that ensure exceptional quality and reliability in adenoviral vector development and manufacturing. At the heart of our purification and analysis workflow are advanced ÄKTA Pure/Avant FPLC and HPLC systems, enabling precise vector purification and sophisticated analysis. The production capability is powered by industrial-scale bioreactor systems (up to 200L) and automated cell culture systems, supported by Wave bioreactors for flexible scale-up operations, enabling efficient and scalable manufacturing from research to pilot scale. For downstream processing, we utilize sophisticated tangential flow filtration (TFF) systems and large-scale chromatography platforms to ensure optimal purification outcomes while maintaining vector integrity. Our analytical strength is further enhanced by cutting-edge flow cytometry and digital PCR systems, enabling precise characterization and quantification of viral vectors. This exceptional combination of advanced equipment is complemented by comprehensive supporting facilities including stability chambers, multiple centrifugation systems, and dedicated quality control instruments. Our integrated platform ensures consistent delivery of high-quality adenoviral vectors that meet the most rigorous standards for research and development applications, while providing the flexibility to accommodate diverse project requirements and scale-up demands.

TRUSTED BY INDUSTRY LEADERS

"Working with the team on our adenoviral vector scale-up project was highly productive. They successfully optimized our production process from 50L to 200L scale while maintaining consistent quality. The physical titer increased from 8E12 to 1.2E13 vp/mL, with >93% purity. Their detailed process documentation and responsive technical support were particularly valuable for our development timeline."

— Dr. Sarah C., Senior Director of Process Development

"We collaborated on developing a helper-dependent adenoviral vector. Their expertise in vector design and optimization was evident throughout the project. The final construct showed excellent expression levels and met all our safety requirements. Particularly impressive was their ability to achieve <0.1% helper virus contamination while maintaining high yields. The comprehensive QC package greatly facilitated our regulatory filing."

— Dr. Michael R., Principal Scientist

"We approached the team with a challenging project requiring specialized adenoviral vectors for RNAi delivery. Their expertise in vector design and process development was evident as they successfully incorporated our complex expression cassette while maintaining vector stability. The final product exceeded our specifications with >90% full capsids and demonstrated excellent knockdown efficiency in our target cells. Their ability to scale up production from 10L to 50L while maintaining product quality was crucial for our program advancement."

— Dr. Emily W., Director of Discovery Research.

Get a Quote Now →

FREQUENTLY ASKED QUESTIONS

What are the available scales for adenovirus production and their typical applications?

We offer multiple production scales to meet different research and development needs:

Research Scale (1-5L): Suitable for proof-of-concept studies and preliminary research
Medium Scale (10-50L): Ideal for preclinical studies and process development
Large Scale (100-200L): Designed for pilot production and scale-up validation

Each scale includes comprehensive process optimization, in-process testing, and detailed documentation. Production can be adjusted based on your specific titer requirements and application needs. We also provide consultation on scale selection based on your downstream applications and quantity requirements.

What starting materials do I need to provide for adenoviral vector development?

For standard vector construction, you need to provide either the transgene sequence information or the ready-to-use transgene plasmid. For sequence information, please provide the complete CDS with detailed annotation. For plasmids, we require 2-5 µg high-quality DNA with concentration ≥100 ng/µL and comprehensive sequence data. If you have specific requirements for promoters, regulatory elements, or vector backbones, please specify in advance. We can also assist with construct design and optimization, including codon optimization and regulatory element selection. All provided materials should be accompanied by detailed documentation including sequence verification, source information, and any relevant safety data.

What quality control parameters do you test for, and what documentation will I receive?

Our comprehensive QC testing package includes:

Identity Testing: Transgene sequencing, restriction mapping
Purity Analysis: SDS-PAGE, SEC-HPLC, residual host cell DNA/protein
Biological Testing: Sterility, endotoxin, mycoplasma
Potency Testing: Physical titer (vp/mL), infectious titer (IFU/mL)
Function Verification: Transgene expression, specific activity

Each batch is delivered with a detailed Certificate of Analysis covering all test results, specifications, and methods. We provide complete documentation including:

Development history and production records
Analytical methods and validation reports
Stability data and storage recommendations
Raw data from key analyses

Additional customized testing can be performed upon request to meet specific requirements.

What viral titers and purity levels can I expect for different applications?

Our typical production achieves the following specifications:

Parameters	Research Grade	Development Grade
Physical Titer	1×10^12-13 vp/mL	>1×10¹³ vp/mL
Infectious Titer	1×10^10-11 IFU/mL	>1×10¹¹ IFU/mL
Purity (SEC-HPLC)	>90%	>95%
Host Cell Protein	<100 ng/mg	<50 ng/mg
Host Cell DNA	<10 ng/dose	<5 ng/dose

Specifications can be customized based on your specific requirements. We implement comprehensive optimization strategies to achieve desired quality targets while maintaining high yields. Each batch undergoes thorough testing to ensure consistent quality and performance.