Adenovirus Vector
Cancer gene therapy requires introducing new genetic material into target cells through an effective vector without toxicity to non-target tissues. Adenoviral vectors are good candidates for direct in vivo gene transfer. The world's first licensed gene therapy product is based on the adenovirus as a gene delivery vector. Creative Biolabs provides the state-of-the-art adenoviral vector with the various design and construction to meet the demand in basic research and preclinical applications.
Adenovirus Vector Construction
Adenoviruses are nonenveloped viruses with an icosahedral protein capsid consisting of a linear double-stranded DNA genome. Since adenovirus vectors cannot be integrated into the host genome, multiple administrations are required. The adenovirus vector is flanked by ITRs, whose inside is a viral packaging signal. Four early transcription elements (E1, E2, E3, E4) are distributed on the genome to regulate replication. Most frequently used and best-characterized adenovirus vectors are based on types 5 and 2 of human species C. Creative Biolabs offers various types of adenovirus vectors with different design and construction for customers to choose from. The gutless is superior to the adenovirus vector in packaging capacity and toxicity.
Fig.1 Adenovirus vector construction for gene therapy
The vector is chemically modified with a polymer after production and purification, so as to eliminate the barrier presented by the vector-host interaction. Here, at Creative Biolabs, PEG and pHPMA are used to modify adenovirus vectors to maintain high-efficiency gene transfer and overcome the barriers that limit clinical applicability. The adenovirus vector is becoming the most widely used gene therapy vector. Different Ad serotypes made with various construction methods can be applied to pancreatic carcinoma, neuroendocrine carcinoma, breast carcinoma, and glioblastoma, etc.
Adenovirus Vector System
- AdEasy system: The recombination efficiency is greatly improved by prokaryotic recombination.
- AdMax system: The eukaryotic adenovirus packaging system modified by Cre / LoxP has increased titer.
Fig.2 Recombinant process of the AdEasy system
Fig.3 Recombinant process of the AdMax system with Cre-loxP
SERVICES
Based on our top technology platform and experienced scientists, Creative Biolabs provides state-of-the-art adenovirus vector construction, characterization, optimization and purification services to meet the demands in basic research and preclinical applications. Our services mainly include the followings:
Features
- Easy to purification and concentration
- Broad host range
- Transient expression
- Strong immunogenicity
- High-efficiency rate of host cell infection of dividing or non-dividing cells
- High-efficiency administration because of relatively higher titer
We also provide recombinant adenovirus vector products containing various promoters and shRNA-encoded genes.
With world-class expertise of viral vector technology, Creative Biolabs provides customized viral vector production and has robust quality control. We offer the highest-quality one-stop adenovirus vector related services for gene delivery, including the design and construction of adenovirus vectors according to different experimental needs. Please feel free to contact us for more details and our scientists will tailor the most reasonable scheme for your projects. We are pleased to serve you.
Reference
- Lee, C. S.; et al. (2017). Adenovirus-mediated gene delivery: potential applications for gene and cell-based therapies in the new era of personalized medicine. Genes & diseases, 4(2), 43-63.
