Creative Biolabs has years of experience in providing protein engineering services for our global customers. With our rich experience and extensive expertise, scientists of Creative Biolabs have developed a novel membrane- assisted isoform immunoassay technology (MAIIA) for measuring protein various isoforms in biological specimens. In combination with our rapid and sensitive technology, we are confident in providing the best protein isoform detection services to meet the specific requirements from our customers.
Protein isoform formation
Proteins are structurally heterogeneous molecules and the same protein can exist in many isoforms (sub-populations). A set of protein isoforms may be present and formed due to alternative splicing, polymorphism and post-translation modifications (PTMs). For examples, actin isoforms are originated from separate genes, because single gene can code for multiple proteins during the mRNA splicing. In addition, a glycoform is an isoform of a protein that differs only with the number or type of attached glycan. Therefore, glycoproteins often have a lot of glycoforms which may result from variation in the carbohydrate structures during the process of glycosylation.
Fig.1 Protein isoforms A, B, and C are formed by alternative splicing.
Importance of protein isoform
Protein isoforms are involved in various stages in a number of pathologies, playing important roles in various biological processes. Therefore, they have potentially been used as biomarkers and therapeutic targets. For example, several specific proteins isoforms are related to thyroid pathological conditions and various cancers, multiple sclerosis and autoimmune diseases. Therefore, it is very important to detect the protein isoforms.
Protein isoforms detection method: MAIIA
The existing separation and detection methods for protein isoforms, such as chromatographic separation and electrophoretic separation, are far too complicated and time-consuming.
Based on the extensive experience in protein engineering, scientists of Creative Biolabs have built up an innovative membrane-assisted isoform immunoassay (MAIIA) method for protein isoform detection. This technique combines the separation of protein isoforms (by ion-exchange or affinity chromatography) with an immunoassay detection method in a disposable membrane device. It is possible to quantify the protein isoforms in a very fast and simple way (<15 min). In addition, it has achieved much higher levels of specificity than the traditional methods. This technique has been successfully applied in measuring carbohydrate-deficient isoforms of the glycoprotein transferrin. Notably, with our MAIIA technology, we are able to resolve and detect other types of post-translational modified proteins even when they occur in low concentration. Our technique has been widely used for rapid testing of clinically interesting protein isoforms.
The advantages of MAIIA technology:
Fig.2 A protein isoform separation.
Creative Biolabs offers the best protein isoform detection services with our advanced technology. Scientists of Creative Biolabs are pleased to share our experience with our global customers. For more detailed information, please feel free to contact us or directly send us an inquiry.