Tumor Organoid and TIL Co-Culture Model for Immuno-Oncology Drug Discovery and Development

TILs (tumor-infiltrating lymphocytes) exhibit specific anti-tumor immune activity and are populations of lymphocytes that penetrate deep inside tumors for immune assault. Creative Biolabs provides co-culture models of tumor organoids and TILs that allow for better characterization of the tumor immune landscape and evaluation of drugs.

Model Building

1. Tumor organoid culture. The source can be either a tumor sample or a tumor cell line.
2. Isolation and amplification of TILs. TILs are isolated from tumor tissue and expanded in vitro. This can be achieved by cutting tumor tissue, digesting and isolating lymphocytes.
3. Co-culture modeling. Optimization of culture conditions and establishment of a co-culture system consisting of amplified TILs and tumor organoids.

Fig 1. Schematic representations of the culture method of patient-derived spheroids and organoids.¹

Applications

Studying the behavioral patterns of TILs in specific tumors.

Initial cell distribution and multiple recombinational analyses in co-culture models were investigated. The study of time-dependent migration patterns of TILs permits simultaneous longitudinal detection of TIL activation and cytokine secretion, which can be used as a key indicator for assessing adaptive responses.

Immunotherapy strategy evaluation.

Through the co-culture model, the dynamic killing effect of TILs on tumor cells can be assessed in real-time, advancing the establishment of an anti-tumor overt TIL strategy.

Immune tolerance studies.

To study tumor immune escape pathways involving TILs, including immune checkpoint regulation and cellular depletion, and to develop personalized strategies to overcome tolerance.

Drug screening.

To simulate the effect of drugs in vivo on the clustered tumor environment of TILs in actual lesions, the degree of infiltration and distribution of TILs in 3D organoid co-culture models were used as predictive indicators to screen effective drugs for targeted modulation.

Tumor Organoid and TILs Co-Culture Model Advantages

• Multiple T-cell receptor clonality.
• Superior tumor-homing ability.
• Lower targeted toxicity.
• Allows amplification of tumor samples from different sources.
• Enables modeling of tumors at different stages.
• Enables separate banking of cancer and paracancerous tissues.

Co-culture models of tumor organoids and autologous or non-autologous immune cells help to overcome the limited information available in traditional models. Creative Biolabs offers a variety of patient-relevant ex vivo platforms for the development of immunotherapeutic agents including co-culture models of tumor organoids and TILs. Please contact us and get a response with details.

Related Sections:

• Tumor Organoid Monoculture Model for Immuno-Oncology Drug Discovery and Development
• Tumor Organoid and Immune Cell Co-culture Model for Immuno-Oncology Drug Discovery and Development