Tumor Organoid and Immune Cell Co-culture Model for Immuno-Oncology Drug Discovery and Development

Limitations of Conventional Models in Immune-Oncology Drug Discovery and Development

Limitations of animal models

Traditional mouse models have been widely used in immune-oncology drug discovery and development. However, animal models cannot fully predict the human immune system's response to drugs due to species differences and the complexity of the immune system, limiting the translational potential of animal models.

Limitations of 2D cell culture

Traditional 2D cell culture models lack the three-dimensional structure and microenvironment of tumors, failing to accurately simulate the interaction between tumor cells (TCs) and immune cells (ICs). Additionally, 2D cell culture models cannot preserve the heterogeneity and phenotypic characteristics of the original tumor, leading to discrepancies between in vitro screening results and in vivo efficacy.

Lack of IC presence

Conventional tumor organoid (TO) models often lack the presence of ICs, while ICs play a critical role in immunotherapy. Therefore, to better understand the interaction and drug response between ICs and TCs, there is a need to develop models that can simultaneously culture ICs and TCs.

Tumor Organoid and Immune Cell Co-culture Model (TOAICCM) at Creative Biolabs

Fig 1. The procedures of cancer organoids establishment and applications of the co-culture system.¹

To overcome these limitations, the TOAICCM has emerged. This model combines the three-dimensional structure of TOs with the presence of ICs, enabling a more accurate simulation of the interactions between ICs and TCs in the tumor microenvironment. By co-cultivating ICs and TCs in the organoid model, it is possible to recapitulate physiological conditions and immune responses more faithfully, thereby better evaluating the efficacy of immune-oncology drugs. Creative Biolabs offers the most comprehensive TOAICCMs:

Advantages of TOAICCM

Recapitulation of the tumor microenvironment

TOs can replicate the complex spatial morphology of the original tumor tissue, including cell-cell and cell-extracellular matrix interactions, providing a better simulation of the tumor microenvironment.

Simulation of IC infiltration and interactions

The co-culture model provides a platform for studying the interactions between ICs and TCs, facilitating research on the activity, infiltration, and modulation of the tumor microenvironment by ICs

Contribution to tumor biology research

This highly simulated tumor immune microenvironment can be used to study characteristics of TCs, such as proliferation, survival, invasiveness, and metastatic potential, enabling a deeper understanding of the biological behavior and regulatory mechanisms of TCs.

Facilitation of drug screening and optimization

The co-culture model can be employed for screening and evaluating candidate immunotherapeutic drugs, more accurately predicting the impact of drugs on IC activity and TC-killing ability, thus assisting in the selection of the most effective drugs and optimization of drug design.

Reasons to Choose Creative Biolabs

Expertise and experience

We possess extensive experience and expertise, particularly in the fields of tumor biology and immunotherapy.

The high degree of customization

We design and construct TOs and IC co-culture models tailored to meet our clients' specific needs and research goals.

Comprehensive technical platform

We have a comprehensive technical platform that enables us to employ various cellular and molecular biology techniques for comprehensive analysis and evaluation.

Accelerated research and development process

By providing more realistic experimental data, we assist clients in selecting the most effective drugs and optimizing drug design, thereby saving time and resources.

By choosing Creative Biolabs as a partner, clients can gain an in-depth understanding of model characteristics and drug effects. Contact us now to embark on an innovative journey in immunotherapy drug research!

Related Sections:

• Tumor Organoid for Immuno-Oncology Drug Discovery and Development
• 3D Spheroid Model for Immuno-Oncology Drug Discovery and Development