Magic™ Custom GPCR Preparation Service

Creative Biolabs currently has integrated a comprehensive service package for GPCR-related studies. Based on our unparalleled Magic™ membrane protein platform as well as extensive experience during the past decades, we provide world-class custom membrane protein production, including GPCR, ion channels, and transporters. We are glad to leverage our professional knowledge and assistance to facilitate their cutting-edge research programs.

Overview of G-protein-coupled Receptors

G-protein-coupled receptors (GPCRs) represent the largest family of cell surface receptors and account for approximately 4% of the protein-coding human genome. They participate in a variety of important physiological functions and play a critical role as indicators of disease with diagnostic and prognostic potential. However, GPCR studies have been hindered by their low natural abundance, inherent structural flexibility and dynamics, and the instability in detergent solutions. With years of experience and state-of-the-art technologies, Creative Biolabs now provides custom GPCR production service based on our versatile Magic™ platform. We tailor custom-oriented strategies to meet every specific demand for downstream applications.

G-protein-coupled receptors (Wikipedia, https://commons.wikimedia.org/wiki/File:PDB_1hzx_7TM_Sketch_Membrane.png)

Strategies of GPCR Production

Cell-based Full-length GPCR expression

Creative Biolabs is capable of producing many different GPCRs using E.coli cells, yeast cells, insect cells, and mammalian cell systems. We will first conduct exhaustive literature research to help you select the most appropriate system, and perform multiple condition screenings/optimization for the best outcome. Using our powerful Magic™ membrane protein production platform, products can be subsequently stabilized in detergent, liposomes, nanodiscs, and VLPs.

Cell-free Full-length GPCR expression

Compared with the cell-based method, the innovative cell-free machinery can provide many advantages like robust expression, small reaction volume, easy purification, and no detergent use. De novo synthesized proteins can be directly reconstituted into various formats including detergents, liposomes, or nanodiscs. Both prokaryotic (E. coli) and eukaryotic (wheat germ, rabbit reticulocyte lysate, insect cell lysate, mammalian cell lysate) are available, from which many GPCRs have been successfully obtained at mg level.

Peptides derived from extracellular and intracellular domains

If the indicated GPCR is structurally characterized, we can just express a well-designed peptide derived from the extracellular/intracellular domain of interest. Instead of full-length GPCRs which can take many efforts to obtain, this plan can be a lot easier and well-compatible for antibody raising or assay use.

GPCR stable cell lines

Creative Biolabs is professional in constructing GPCR overexpressing stable cell lines, including specialty cell lines for function study use. We have many GPCR-stable cell lines with high expression levels of up to 5 million per cell, which can be directly utilized for membrane isolation, whole-cell immunization, and various assays.

In Creative Biolabs, we are always challenging ourselves to best meet our clients' satisfaction and expectations. Our Magic™ membrane protein platform has benefited numerous researchers worldwide by providing high-quality GPCR products. Function characterization/validation can also be performed for strict QC. Please contact us for more information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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