Creative Biolabs currently has integrated a comprehensive service package for GPCR-related studies. Based on our unparalleled Magic™ membrane protein platform as well as extensive experience during the past decades, we provide world-class custom GPCR, ion channels and transporters production service to our global clients. We are glad to leverage our professional knowledge and assistance to facilitate their cutting-edge research programs.
Fig 1. Rhodopsin-Like GPCR
G-protein-coupled receptors (GPCR), aka 7 transmembrane receptors, represent the largest family of cell surface receptors and account for approximately 4% of the protein-coding human genome. They participate in a variety of important physiological functions (e.g. neurotransmitters and hormone receptors), thus are playing a critical role as indicators of disease with diagnostic and prognostic potential. In fact, GPCR targeting agents constitute over 50% of all current drugs in market, including more than a quarter of the 100 top-selling drugs.
However, GPCR studies have been hindered by their low natural abundance, inherent structural flexibility and dynamics, and the instability in detergent solutions. The establishment of robust, reliable and cost-effective production of GPCR proteins has always been a primary focus in various fields like drug discovery, structural analysis, assay development, etc. With years of experience and state-of-the-art technologies, Creative Biolabs now provides custom GPCR production service based on our versatile Magic™ platform. We tailor custom-oriented strategies to meet every specific demand for downstream applications:
Creative Biolabs is capable of producing many different GPCRs using yeast cell, insect cell, and mammalian cell system. We will first conduct exhaustive literature research to help you select the most appropriate system, and perform multiple condition screenings/optimization for the best outcome. Using our powerful detergent screening platform & lipid screening platform, products can be subsequently stabilized in detergent micelles, liposomes, nanodisc, and polymers.
Compared with the cell-based method, the innovative cell-free machinery can provide many advantages like robust expression, small reaction volume, easy purification and no detergent use. De novo synthesized proteins can be directly reconstituted into various formats including detergents, liposomes or nanodiscs. Both prokaryotic (E. coli) and eukaryotic (wheat germ, rabbit reticulocyte lysate, insect cell lysate, CHO/HEK lysate) are available, from which many GPCRs have been successfully obtained in mg level.
Virus-like particles (VLP) can be employed to express heterologous membrane proteins by modifications of viral genome. VLP-derived lipoparticle is another alternative for integral membrane incorporation. This approach is very suitable for immunogen preparation.
Peptide derived from extracellular and intracellular domains
If the indicated GPCR is structurally characterized, we can just express a well-designed peptide derived from the extracellular/intracellular domain of interest. Instead of full-length GPCRs which can take many efforts to obtain, this plan can be a lot easier and well-compatible for antibody raising or assay use.
GPCR stable cell line and products
Creative Biolabs is professional in constructing GPCR overexpressing stable cell lines, including specialty cell lines for function study use (i.e. internalization, whole fluorescent, receptor function screening cell lines). We have over 100 GPCR stable cell lines (CHO/HEK background) with high expression levels up to 5 million per cell, which can be directly utilized for membrane isolation, whole-cell immunization, various assays.
Particularly, we also offer well-characterized, ready-to-use GPCR products reconstituted in detergent micelles, liposomes or nanodiscs.
In Creative Biolabs, we are always challenging ourselves to best meet our clients’ satisfaction and expectations. Our Magic™ membrane protein platform has benefited numerous researchers worldwide by providing high-quality GPCR products. Function characterization/validation can also be performed for strict QC. We can provide custom GPCR synthesis service as an integral step in our anti-GPCR antibody development/antibody functional assay service package. Please contact us for more information.