Creative Biolabs now provides integral service packages of membrane transporter protein preparation for our worldwide customers. Equipped with state-of-the-art Magic™ membrane protein platform, our scientists have successfully synthesized, purified and reconstituted a broach range of membrane protein targets, including GPCRs, ion channels, transporters as well as viral coat proteins.
In a broad sense, transporter proteins represent a large membrane protein family involved in the movement of ions, small molecules, and macromolecules across the plasma membrane. As is known, biological phospholipid bilayer is impermeable to many substances, especially many drug reagents, transporters thus play critical roles in cell survival, functioning, pharmacodynamics/pharmacokinetics and many other aspects. They are mostly integral membrane proteins multi-spanning the membrane across which the transporter target molecules. The mechanisms of transportation include active transport (against concentration gradient using energy input, such as glucose and amino acids) and facilitated diffusion (or passive transport, down the concentration gradient, no energy required).
Transporter proteins can be further characterized into 3 different types as follow:
Fig 1. Rhodopsin-Like GPCR
Transporter dysfunction is closely correlated with many inherited or acquired diseases, e.g. cystinuria. Besides, drug efflux pumps can also lead to severe drug resistance in cancer cells, which is of increasing concern in oncology treatment. However, the extremely diverse family members, complicated structure, and relatively low expression levels greatly impede the progress of membrane transporter studies. After years of commitment in this field, Creative Biolabs has established a robust, reliable and cost-competitive transporter preparation platform. We offer a broach range of options from which you can always find the best match for your project.
We can generate the target transporter using many conventional cell-based systems including:
Using our powerful detergent screening platform & lipid screening platform, protein products can be subsequently purified and incorporated into various forms, like detergent micelles, liposomes, nanodisc and polymers for further validation.
Sometimes cell-free machinery is more efficient than cell-based formats especially for complex targets like transporters. Problematic issues like misfolding, detergent impair and inclusion bodies will be less of concern in cell-free systems. In particular, we recommend our featured wheat germ system, which has proved good utility in expressing many ion channels and transporters.
Native membrane proteins can be directly captured using spontaneous assembled viral core proteins, forming high-density membrane protein-displaying lipoparticles. No need for risky detergent use or labor-intensive purification process. VLP-derived lipoparticle is very suitable for immunization and antibody selection.
Peptide derived from extracellular and intracellular domains
If structural information is accessible for the target transporter protein, we can design and express the extracellular or intracellular domain directly. In contrary to full-length multipass, it will be much easier and less risky to get peptide loops of interest. We have raised many anti-membrane protein antibodies with high specificity and affinity using this strategy.
Transporter stable cell line and products
Creative Biolabs has rich experience in establishing membrane protein overexpressing stable cell lines, including special cell lines for function investigation use (like internalization, whole fluorescent, receptor function screening cell lines). Currently, we have a wide range of ready-to-use stable cell lines with high expression levels.
Creative Biolabs is always ready to leverage our expertise and dedication to help facilitate our customers’ research programs. Our tailored service has earned us broad reputation for the successful accomplishment of many challenging transporter protein projects. Of note, strict QC/functionality validations can also be conducted. We also provide anti-transporter antibody development/antibody functional assay service. Please feel free to inquire us for more details.