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Rat Hybridoma Generation

Creative Biolabs has established a unique platform to develop high-affinity monoclonal antibodies in rats. Compared with commonly used mouse antibodies, rat antibodies do not have the background cross-reaction problems in immune-detection of antigens out of a mouse background, such as a mouse antigen from a mouse animal model. Another reason is that antibodies against mouse protein are difficult to be raised in mice themselves. Therefore, antibodies from other species have to be considered and rat monoclonal antibodies are a good choice.

However, Rat monoclonal antibodies are much more difficult to develop compared with mouse monoclonal antibodies due to the lack of a stable fusion partner cell line for rat hybridoma generation. Together with Creative Diagnostics, Creative Biolabs has developed a proprietary hybridoma-generation platform for efficient development of high-affinity and high-specificity rat monoclonal antibodies. Our Rat Monoclonal Antibody platform allows us to offer the most comprehensive one-stop custom antibody development services in the industry.

Features of our rat hybridoma production services:

1. Picomolar affinity and high specificity
2. Excellent results even for difficult antigens
3. Ability to recognize subtle changes such as post-translational modifications
4. Novel epitope recognition
5. Cross-reactive antibodies recognizing human proteins and mouse orthologs
6. Excellent IHC results with formalin fixed paraffin embedded tissue
7. Ability to generate large panels of bioactive antibodies

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