Dithiobis(succinimidyl propionate) (DSP) Homobifunctional Crosslinker (CAT#: OLY-1023-YS141)

Description

DSP, also known as Lomant's Reagent, is a homobifunctional, amine-reactive, and cleavable crosslinker. It features two N-hydroxysuccinimide (NHS) ester groups that react with primary amines (lysines) at pH 7.0-9.0. The key feature is that the 12-angstrom spacer arm contains a central disulfide bond. This bond is stable during conjugation but can be easily cleaved using reducing agents like DTT or TCEP. This cleavability makes DSP ideal for two-stage applications, such as identifying protein-protein interactions (e.g., separating crosslinked complexes on a 2D-PAGE gel).

Molecular Weight

404.41

Purity

>97.0%

Formula

C₁₄H₁₆N₂O₈S₂

Product Type

Homobifunctional Crosslinkers

Group 1

NHS

Group 2

NHS

Form (20 deg.C)

Solid

Storage Condition

Store under inert gas, 2-8°C

Molecular Structure