Selective and rapid detection of biomarkers is of utmost importance for early-stage diagnosis to prevent fatal diseases and infections. The role of lysozyme has been illustrated to be especially important in the human immune system to prevent several bacterial infections and other chronic diseases. Having accumulated rich experience in biomarker detection, Creative Biolabs provides aptamer development and biosensor construction services to meet your lysozyme detection requirements.
Lysozyme, a 14.3 kDa protein ubiquitously present in many organisms, has been identified with a variety of functions including antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods, or a model of amyloid-type protein aggregation. Relatively small size and simplicity make it an excellent model analyte for novel methods in protein detection. Studies reported that antibodies against citrullinated variants of lysozyme are present in patients with rheumatoid arthritis (RA), which highlights the potential of lysozyme as a clinical index of these diseases.
The classical method for lysozyme determination is based on the ability of lysozyme to clear a suspension of Micrococcus lysodeikticus cells. Other methods based on the quantification of the protein amount and not its enzymatic activity include immunoenzymatic, electrophoretic, and chromatographic methods. However, these lysozyme detection methods are expensive, time-consuming, or cannot be applied for fast in-situ quantification.
Electrochemical and optical sensors have attracted increasing attention due to their versatility and ability to reduce the disadvantages of traditional methods. Next to these years, aptamer-based analytical methods have been developed towards lysozyme detection. The positive nature of lysozyme renders the protein favorable for specific binding on DNA aptamers. Various electrochemical aptasensors, based on different assay formats, aptamer selection strategies, and detection methods have been developed for lysozyme sensing in various samples.
Fig.1 Aptasensors for lysozyme detection. (Melinte, 2021)
Creative Biolabs performs the selection of aptamers for lysozyme with high selectivity and affinity using capillary electrophoresis-SELEX (CE-SELEX). Then, the selected aptamers will be characterized for binding affinity using affinity capillary electrophoresis, fluorescence anisotropy, and surface plasmon resonance (SPR). Aptamers with nanomolar dissociation constants may function as biorecognition molecules to develop biosensors for the detection and quantification of lysozyme in food and pharmaceuticals to ensure compliance with allergen labeling and improve consumer protection.
If you are interested in aptamers development for lysozyme detection, please feel free to contact us for more information or a detailed quotation.
Reference
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