Creative Biolabs is a biotechnology company that focuses on the development of sensitive and accurate IVD assays to advance disease diagnosis. Given the validity of restriction enzyme analysis in disease diagnosis, we support the design and development of assays for DNA restriction enzyme analysis, facilitating its application in the IVD field.
Restriction enzymes, also known as restriction endonucleases, are DNA-cutting enzymes isolated from bacteria. These enzymes are thought to play an important role in host defense by cutting and destroying viruses or other foreign DNA. Restriction endonuclease recognizes specific palindromic DNA sequences and cuts the double-stranded DNA into fragments at specific recognition sites. These sites are called restriction sites, normally ranging from 4-8 base pairs in length. Now there are many different types of restriction enzymes that have been identified such as EcoRI, BamHI, and HindIII. Each type recognizes a different restriction site. For example, BamH1 specially recognizes the sequence of GGATCC and EcoRI specially recognizes the sequence of GGATCC. Restriction enzymes have a variety of applications in biological fields such as molecular cloning, single-nucleotide polymorphisms (SNPs) identification, and DNA fingerprinting. Furthermore, DNA restriction enzyme analysis is increasingly used as a tool for the diagnosis of some genetic disorders.
Sickle cell anemia is a genetic disease of the red blood cells (RBCs) that is caused by a single-base mutation of the β-globin gene. In normal people, the β-globin gene sequence is CCTGAGGAG, while in sickle cell anemia, the β-globin gene sequence is CCTGTGGAG. The single-base mutation (A to T) in the β-globin gene destroys the recognition site of the Mstll. Therefore, the mutation can be detected by using restriction enzyme Mstll to cut DNA fragments in and around β-globin gene, followed by performing a hybridization with a cloned β-globin DNA probe.
Creative Biolabs is an expert in the field of IVD development. We have developed and optimized in-house IVD development platforms, allowing detection of disease markers from multiple levels involving gene, protein, cell, and tissue. On the level of the gene, we have established a variety of powerful technologies such as PCR, next-generation sequencing, microarray, and DNA restriction enzyme analysis. We are capable of supporting one-stop IVD development services from scheme design, implementation to data delivery. For DNA restriction enzyme analysis, we provide a full range of testing design and development services for our clients, including DNA isolation, restriction enzyme digestion, gel electrophoresis, probe design and synthesis, and hybridization. We also support customized services according to your special requirements.
Fig.1 Steps in restriction endonuclease analysis of the DNA.
If you are interested in our services, please don't hesitate to contact us for more details. We are thrilled to become your reliable partner to develop novel specific IVD assays for disease diagnosis research.
Reference
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