The sequence of nucleic acids in polynucleotide chains ultimately contains the information for the hereditary and biochemical properties of terrestrial life, which has been an important reference for in vitro diagnostics (IVD). Therefore, the ability to measure or infer DNA sequences is imperative to biological research, especially in the IVD field. With our deeply-rooted IVD expertise and abundant experience, Creative Biolabs provides DNA sequencing services to accelerate the development of your IVD projects.

Introduction of DNA Sequencing

DNA sequencing is the process of determining the nucleic acid sequence (the order of nucleotides in DNA). It is used to determine the order of the four bases: adenine (A), guanine (G), cytosine (C), and thymine (T). The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Knowledge of DNA sequences has become indispensable for basic biological research and has been applied in numerous fields such as biotechnology, forensic biology, virology, and biological systematics. There are many kinds of DNA sequencing that have been developed, such as shotgun sequencing, sanger sequencing, next-generation sequencing (NGS), single-cell sequencing (SCS), etc.

Three generations of human genome sequencing technology.Fig.1 Three generations of human genome sequencing technology. (Dewey, 2012)

Next-Generation Sequencing (NGS)

NGS, also known as high-throughput sequencing, is used to sequence DNA and RNA rapidly and cost-effectively. NGS performs a progressively important role in cancer diagnosis and prognosis. NGS examinations for the prostate, breast, ovarian, pancreatic, hematological malignancies and others can predict new tumor-associated antigens (TAAs). At the same time, scientists utilize NGS technology to diagnose genes related to hereditary cancers.

Single-Cell Sequencing (SCS)

Single-cell sequencing examines the sequence information from individual cells with optimized NGS technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. Scientists find that the genetic alterations of the cancer stem cell (CSC) probably can be effectively masked by single-cell sequencing. Compare to mixed cell sequencing, SCS could unravel key points much clearer in cancer biology that is difficult to address with bulk tumor sequencing. Thus, this method will be greatly helpful for scientists' understanding of initiation, progression, invasion, metastasis, resistance, and recurrence in cancer, subsequently guiding a more efficient early diagnosis and targeted therapy in cancer therapies in the clinic.

WGA and WTA Methods for Single Cell Sequencing.Fig.2 WGA and WTA Methods for Single-Cell Sequencing. (Wang, 2015)

Featured DNA Sequencing Services

  • Maxam-Gilbert sequencing
  • Chain-termination methods
  • Large-scale sequencing and de novo sequencing
  • Shotgun sequencing
  • Single-molecule real-time sequencing
  • Ion semiconductor
  • Sequencing by synthesis
  • Combinatorial probe anchor synthesis
  • Nanopore sequencing
  • Chain termination (sanger sequencing)
  • Whole-genome sequencing
  • Massive sequencing technologies
  • Genomic sequencing
  • Subgenomic sequencing

Why Us?

(1) We have advanced testing instruments and professional operators to provide you with the most rigorous sequencing results.

(2) Our experts will communicate with you in detail to understand your project needs and provide personalized services.

(3) Our efficient testing service can save you time and cost.

Creative Biolabs has the first-class sequencing capability to provide a full range of sequencing services for customers in the IVD field. With advanced genomics research strength, we will develop the best solutions to provide fast, accurate and reliable DNA sequencing services. By the way, we also provide tailored sequencing solutions to meet your needs. If you are interested in our DNA sequencing, please contact us.

References

  1. Dewey, F. E.; et al. DNA Sequencing Clinical Applications of New DNA Sequencing Technologies. Circulation. 2012, 125(7): 931-944.
  2. Wang, Y.; Navin, N. Advances and Applications of Single-Cell Sequencing Technologies. Molecular Cell. 2015, 58(4): 598-609.

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