Creative Biolabs, a world-leading service provider in the field of antibody discovery and generation, is proud to launch our high-quality in vitro diagnostic (IVD) antibody & immunoassay development services to global clients. Our services are customized toward a variety of disease biomarkers. Especially, we introduce 8-OHDG as a potential diagnostic marker of diseases.
8-hydroxy-2'-deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is one of the major forms of free radical-induced oxidative lesions in nuclear and mitochondrial DNA. In other words, 8-OHdG is a product of ROS attacking DNA. The levels of 8-OHdG are increased in both mitochondrial DNA and nuclear DNA with age. Furthermore, the increasing levels of 8-OHdG likely contribute to carcinogenesis by regulation of gene expression and induction of mutations. 8-OHdG has been shown to regulate epigenetic alteration such as CpG islands in a promoter region of a gene which could reduce the expression of the tumor suppressor gene and contribute to carcinogenesis. Currently, 8-OHdG has been widely used as a biomarker for oxidative stress and carcinogenesis. Also, 8-OHdG has a key role during the formation of memory by promoting thousands of rapid demethylations of methylated cytosines in CpG sites.
Fig.1 (a) Deoxyguanosine structure ; (b) 8-OHdG structure, deoxyguanosine modification.1
The biological significance of alterations in 8-OHdG levels has been studied in multiple diseases. Elevated levels of 8-OHdG have been reported to be correlated with clinical outcomes of stroke. Besides, serum 8-OHdG was identified as a diagnostic marker for nonalcoholic steatohepatitis (NASH) when comparing the serum 8-OHdG levels in healthy controls and patients with nonalcoholic fatty liver (NAFL) and NASH. Moreover, increased 8-OHdG levels are closely related to the accumulation of mutations triggering carcinogenesis. Studies have reported that a high 8-OHdG concentration in leukocyte DNA was associated with advanced age and poor prognosis of patients with serous ovarian cancer (SOC) in comparison to controls. Also, when comparing the blood levels of 8-OHdG in women with benign breast tumors, breast cancers, and healthy controls, researchers have found a significant increase of serum levels of 8-OHdG in patients with breast cancer and that 8-OHdG can be used as a potential noninvasive biomarker for early detection of breast cancer.
Quantification of 8-OHdG can be carried out with different methods, including antibody-based immunoassays, LC-MS/MS, and high-performance liquid chromatography (HPLC). At Creative Biolabs, we offer custom-tailored IVD antibody and immunoassay development services targeting various disease biomarkers, such as 8-OHdG. We know the importance of antibodies with high specificity and sensitivity to develop reliable kits. With years of experience, we offer services covering the whole process of kit development. For more detailed information about what we offer for each stage of the development process, please click the links below:
Please feel free to contact us for more information and discuss your project needs.
References
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