Creative Biolabs, a world-leading service provider in the field of in vitro diagnostics (IVDs), is very proud of providing high-quality IVD antibody development services to facilitate the diagnosis of different diseases. Currently, we offer nestin-specific IVD antibody development services to aid in the prognosis of breast cancer.

Introduction of Nestin

Nestin (neuronal stem cell protein), encoded by the NES gene in humans, is a member of the class VI family of intermediate filament proteins. These intermediate filament proteins are expressed mostly in nerve cells where they are involved in the radial growth of the axon. Nestin is a major cytoskeletal protein expressed by neuronal precursors in the mammalian central nervous system (CNS). It has been shown to play a role in the regulation of the assembly and disassembly of intermediate filaments which is involved in remodeling of the cell together with other structural proteins. Furthermore, nestin expression has been widely used as a marker for multipotent neural stem cells (NSCs). Nestin knockout mice show embryonic lethality, with neuroepithelium of the developing neural tube displaying significantly fewer NSCs and much higher levels of apoptosis. These findings suggest that nestin is essential for the proper survival and self-renewal of NSCs.

Domain structure of the human nestin protein. Fig.1 Domain structure of the human nestin protein. (Neradil, J., 2015)

Nestin Marker for Breast Cancer

A number of studies have reported that nestin plays a role in the pathology of malignant diseases and is expressed in transformed cells of various human malignancies, suggesting a relationship between nestin expression and the clinical course of some diseases. Nestin has been shown to be expressed in breast cancer stem cells and newly-formed tumor vessels. It is involved in tumor invasion and metastasis. In younger patients with breast cancer, nestin expression is significantly higher. In more aggressive breast cancer cell lines (MDA-MB-231 and BO2), the level of nestin protein is higher. Moreover, nestin immunohistochemical positivity is associated with poor clinical outcomes and decreased survival rates of breast cancer, suggesting nestin is a potential biomarker for breast cancer prognosis.

IVD Antibody Development Services Targeting Nestin Marker

Breast cancer is the second most common malignant cancer in women and the second major cause of cancer death in the woman. The improvement of disease detection and treatment methods can dramatically increase patient survival rates, which largely relies on the discovery of disease-specific biomarkers. Nestin is one of the potential biomarkers for breast cancer diagnosis and prognosis. Creative Biolabs now offers high-quality antibody development services against nestin marker. Moreover, we offer antibody-based immunoassay development services, which are commonly used sensitive and rapid methods to detect key biomarkers specific to breast cancer. With our versatile IVD platform, Creative Biolabs is proud to develop novel nestin-specific antibody from scratch to commercial IVD kit (we can also start with provided antibody candidates).

As a pioneer in the field of IVD antibody development, Creative Biolabs is confident in providing the best services of antibody development, covering different antibody formats and multiple disease markers. Please feel free to contact us for more information.

Reference

  1. Neradil, J., (2015). “Nestin as a marker of cancer stem cells.” Cancer Science, 106(7), 803-811.


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