Creative Biolabs stands as a recognized leader in providing high-quality antigens and antibody conjugation services for in vitro diagnostic applications. Leveraging our extensive experience and advanced technologies, we are now pleased to offer oligonucleotide conjugation services for specific antibody/oligonucleotide compounds.
Oligonucleotide drugs have long faced challenges related to inadequate pharmacokinetic properties and poor cellular uptake. Covalent linkage to various ligands that target specific tissues, enhance cellular uptake, or influence biological distribution is considered an attractive possibility for advancing the application of oligonucleotides. Oligonucleotide-antibodies provide a more efficient and precise option for the detection of low-abundance proteins, widely employed across diagnostic and therapeutic applications.
Fig 1. Oligonucleotide-antibody conjugation.1
With years of dedicated expertise in the field of IVD antibody development, Creative Biolabs is proud to offer high-quality oligonucleotide conjugation services, serving as a sensitive method for protein detection and quantification. Our robust technical platform and professional development team are poised to significantly advance your project development:
Supported by a team of scientists and experts in the field of IVD antibody development, Creative Biolabs offers globally integrated antigen and antibody conjugation services to meet the specific requirements of each client. With rich experience and advanced technologies, we aim to provide oligonucleotide conjugation services in the most optimal and cost-effective manner.
If you are interested in our services and would like more detailed information, please feel free to contact us for personalized technical support. Our project managers will promptly connect with you to help design a tailored solution to meet your specific needs.
Title: Non‑specific interactions of antibody‑oligonucleotide conjugates with living cells
Research Objective:
The research aims to observe the impact of hydrophilic and charged payload conjugation on antibody functionality, particularly the alterations in binding affinity and cell selectivity post-antibody-oligonucleotide conjugation.
Research Methods:
Researchers synthesized a series of fluorescein-labeled single-chain and double-chain antibody-oligonucleotide conjugates. Time-resolved analysis using ligand binding assays was employed to evaluate the interaction of conjugates with specific cell lines MDA-MB-231 (HER2−) and SK-BR-3 (HER2+).
Research Findings:
Research findings indicate that oligonucleotides represent a particularly effective payload, potentially influencing their binding characteristics post-antibody conjugation. Conjugation of single-chain oligonucleotides with proteins induces non-specific interactions consistent with the cell surface, whereas double-chain oligonucleotides exhibit minimal impact. Therefore, in the development of antibody-oligonucleotide conjugates for various applications, careful consideration of their structure and preparation strategy is imperative.
Fig.2 Association rate constants.2
References
For Research Use Only.
