3D Ex Vivo Human LPS-Induced Acne Skin (LPS) Model Introduction

Creative Biolabs has successfully developed 3D ex vivo human LPS-induced acne skin model for researchers and drug developers in the field of dermatology.

Lipopolysaccharide (LPS) and Acne

Acne vulgaris can be a severe skin condition that is associated with the colonization of hair follicles by commensal bacteria Propionibacterium acnes and inflammation. P. acnes is believed to be the causative agent of acne inflammation, but the pathways involved in this process are not yet fully understood. LPS is a potent endotoxin derived from the outer membrane of Gram-negative bacteria. When applied to the skin, it has been shown to elicit a strong inflammatory response that resembles acne.

Immune cells in the sebaceous gland microenvironment of acne patients. Fig 1. Immune cells in acne skin.¹

Why Choose 3D Ex Vivo Human LPS-Induced Acne Skin Model

Traditional Acne Model

Traditionally, the study of acne has relied on animal models and various in vitro models that include sebaceous gland cells, outer root sheath cells, and others. While these models can partially reflect certain features of acne vulgaris, they still have significant limitations. In fact, up to 90% of drug development experiments fail at the clinical stage due to the imperfect nature of early research models. Animal models have significant differences in skin structure and composition compared to human skin with extreme ethical concerns, while in vitro cell culture systems are often limited in their ability to reproduce complex biological functions such as immune response and bacterial growth.

3D Ex Vivo Human LPS-Induced Acne Skin Model

By subjecting healthy skin extracted from residual surgical tissue to LPS stimulation, we have successfully constructed an inducible acne model that offers several advantages over traditional research models, including:

- Greater accuracy: Our model replicates the 3D structure of human skin, allowing for more accurate and relevant results.

- Reproducibility: We ensure that each sample is treated consistently.

- High-throughput: Each skin tissue sample undergo up to 30 biopsy samplings, providing ample material to meet any testing needs you may have.

- Efficiency: Using our model can save time and resources compared to traditional models.

Our Services

Our LPS-induced acne skin model has been utilized for numerous studies to predict the activity of new compounds. As part of our services, we can offer the complete sampling, culturing, and testing process. However, if preferred, you are free to choose the exit point of the process and complete downstream projects in your own facility.

Fig 4. Workflow and analysis of induced psoriasis skin. (Creative Biolabs)

What Do We Provide?

Creative Biolabs provides 3D ex vivo human LPS-induced acne skin model to promote global researchers' projects. Our model is an excellent tool to evaluate whether test compounds will reduce the release of inflammatory cytokines in acne. Particularly, you can customize any form of endpoint analysis you expect as part of our service. Moreover, we have developed induced psoriasis skin (Th17) model, induced atopic dermatitis skin model, and induced inflamed skin model for global researchers. With years of experience, we ensure that our well-processed tissue samples are preserved at the highest quality. Do not hesitate to contact us and consult with our experts to discuss your specific research needs.

Reference

Firlej, Ewelina. et al. "The role of skin immune system in acne." Journal of Clinical Medicine 11.6 (2022): 1579. Distributed under Open Access license CC BY 4.0, the original image's title was changed to "Immune cells in acne skin".

Research Model

3D Biology Based Biomarker Discovery

3D Biology Based Drug Discovery and Development

3D Biology Based Toxicity Evaluation

Supporting Assays

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