This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-CMV-Sec/SPI-CD79α-CD28-MBP) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | 243UET2.2.4 |
Host | Mouse |
Targeting Cell Type | SU-DHL-10 |
Reporter gene | GFP |
Promoter | CMV |
Signal peptides | Sec/SPI |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | MBP |
Linkers | Flexible Linker |
Co-Receptors | CD22 |
Vector Type | Lentivirus |
Vector Name | BGpCDH2 |
Class | 2 single-Cas |
Type | II |
Subtype | A, B, C |
Effector | Cas12a (Cpf1) |
DNA Type | dsDNA |
Nuclease domains | HD/Cas3 |
TracrRNA requirement | No |
PAM/PFS | 5'PFS: non-C; 3'PFS:NAN/NNA |
Insertion | Stat5b |
Construction | GFP-CMV-CD79α-Cas12a (Cpf1)-dsDNA-Stat5b |
Target | RYK |
Alternative Names | RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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