RYK mAb (243UET2.2.4) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR16)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-CMV-Sec/SPI-CD79α-CD28-MBP) and CRISPR-Cas (Cas12a (Cpf1)-dsDNA-HD/Cas3-5'PFS: non-C; 3'PFS:NAN/NNA-Stat5b) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone 243UET2.2.4
Host Mouse
Targeting Cell Type SU-DHL-10
Reporter gene GFP
Promoter CMV
Signal peptides Sec/SPI
Signaling domains CD79α
TM domains CD28
Detection tags MBP
Linkers Flexible Linker
Co-Receptors CD22
Vector Type Lentivirus
Vector Name BGpCDH2
Class 2 single-Cas
Type II
Subtype A, B, C
Effector Cas12a (Cpf1)
DNA Type dsDNA
Nuclease domains HD/Cas3
TracrRNA requirement No
PAM/PFS 5'PFS: non-C; 3'PFS:NAN/NNA
Insertion Stat5b
Construction GFP-CMV-CD79α-Cas12a (Cpf1)-dsDNA-Stat5b

Target Information

Target RYK
Alternative Names RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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