This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-MNDU3-Sec/SPI-CD79α-CD8-CBP) and CRISPR-Cas (Cas12a (Cpf1)-ssRNA-NNGRRT-Wap) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | ATKll.4 |
Host | Humanized |
Targeting Cell Type | Raji |
Reporter gene | RFP |
Promoter | MNDU3 |
Signal peptides | Sec/SPI |
Signaling domains | CD79α |
TM domains | CD8 |
Detection tags | CBP |
Linkers | SEG |
Co-Receptors | LILRB |
Vector Type | Baculovirus-AAV |
Vector Name | BGpCDH4 |
Class | 2 single-Cas |
Type | III |
Subtype | B |
Effector | Cas12a (Cpf1) |
DNA Type | ssRNA |
Nuclease domains | Unknown |
TracrRNA requirement | YES |
PAM/PFS | NNGRRT |
Insertion | Wap |
Construction | RFP-MNDU3-CD79α-Cas12a (Cpf1)-ssRNA-Wap |
Target | RYK |
Alternative Names | RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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