This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (CAT-MNDU3-Tat/SPI-CD79α-CD28-Myc) and CRISPR-Cas (Cas12c (C2c3)-dsDNA-RuvC-NGG-Csn) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | ATK5.5 |
Host | Humanized |
Targeting Cell Type | Farage-Luc2 |
Reporter gene | CAT |
Promoter | MNDU3 |
Signal peptides | Tat/SPI |
Signaling domains | CD79α |
TM domains | CD28 |
Detection tags | Myc |
Linkers | Flexible Linker |
Co-Receptors | LILRB |
Vector Type | Adenoviruses |
Vector Name | BGpCDH3 |
Class | 1 multi-Cas |
Type | IV |
Subtype | A, B, C, D, E, F |
Effector | Cas12c (C2c3) |
DNA Type | dsDNA |
Nuclease domains | RuvC |
TracrRNA requirement | No |
PAM/PFS | NGG |
Insertion | Csn |
Construction | CAT-MNDU3-CD79α-Cas12c (C2c3)-dsDNA-Csn |
Target | RYK |
Alternative Names | RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.