This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (RFP-SV40-Tat/SPI-CD79α-CD8-His) and CRISPR-Cas (Cas13c (C2c7)-RNA-HD/Cas3-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | 484834 |
Host | Mouse |
Targeting Cell Type | DG-75 [D.G.-75] |
Reporter gene | RFP |
Promoter | SV40 |
Signal peptides | Tat/SPI |
Signaling domains | CD79α |
TM domains | CD8 |
Detection tags | His |
Linkers | Short Flexible Linker |
Co-Receptors | CD19 |
Vector Type | AAV |
Vector Name | BGpCDH1 |
Class | 1 multi-Cas |
Type | VI |
Subtype | A, B, C, D |
Effector | Cas13c (C2c7) |
DNA Type | RNA |
Nuclease domains | HD/Cas3 |
TracrRNA requirement | No |
PAM/PFS | - |
Insertion | Stat5a |
Construction | RFP-SV40-CD79α-Cas13c (C2c7)-RNA-Stat5a |
Target | RYK |
Alternative Names | RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
Certificate of Analysis Lookup
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