RYK mAb (RWRU1) B Cell Engineering Specific Plasmid (CAT#: BGS-1223-CR11)

This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-mPGK-Sec/SPIII-CD79β-CD28-MBP) and CRISPR-Cas (Cas12b (C2c1)-RNA-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.

Datasheet

Specifications

Clone RWRU1
Host Human
Targeting Cell Type P3HR-1
Reporter gene GFP
Promoter mPGK
Signal peptides Sec/SPIII
Signaling domains CD79β
TM domains CD28
Detection tags MBP
Linkers Thioredoxin with polylinker
Co-Receptors CD22
Vector Type Lentivirus
Vector Name BGpCDH2
Class 1 multi-Cas
Type VI
Subtype A, B, C, D, E, F
Effector Cas12b (C2c1)
DNA Type RNA
Nuclease domains Unknown
TracrRNA requirement YES
PAM/PFS -
Insertion Stat5a
Construction GFP-mPGK-CD79β-Cas12b (C2c1)-RNA-Stat5a

Target Information

Target RYK
Alternative Names RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase

Product Properties

Format Liquid/lyophilized
Shipping It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs.
Handling Advice No special measures are necessary. Keep away from food, drink and animal feedingstuffs.
Storage Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C.
COA

Certificate of Analysis Lookup

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