This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (GFP-mPGK-Sec/SPIII-CD79β-CD28-MBP) and CRISPR-Cas (Cas12b (C2c1)-RNA-Stat5a) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
Clone | RWRU1 |
Host | Human |
Targeting Cell Type | P3HR-1 |
Reporter gene | GFP |
Promoter | mPGK |
Signal peptides | Sec/SPIII |
Signaling domains | CD79β |
TM domains | CD28 |
Detection tags | MBP |
Linkers | Thioredoxin with polylinker |
Co-Receptors | CD22 |
Vector Type | Lentivirus |
Vector Name | BGpCDH2 |
Class | 1 multi-Cas |
Type | VI |
Subtype | A, B, C, D, E, F |
Effector | Cas12b (C2c1) |
DNA Type | RNA |
Nuclease domains | Unknown |
TracrRNA requirement | YES |
PAM/PFS | - |
Insertion | Stat5a |
Construction | GFP-mPGK-CD79β-Cas12b (C2c1)-RNA-Stat5a |
Target | RYK |
Alternative Names | RYK; D3S3195; JTK5; JTK5A; RYK1; receptor-like tyrosine kinase; receptor like tyrosine kinase |
Format | Liquid/lyophilized |
Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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