N-Succinimidyl Iodoacetate Heterobifunctional Crosslinker (CAT#: OLY-1023-YS84)

Description

SIA is a classic heterobifunctional, non-cleavable crosslinker. It features an amine-reactive N-hydroxysuccinimide (NHS) ester and a sulfhydryl-reactive iodoacetyl group. The NHS ester reacts efficiently with primary amines (lysines) at pH 7.0-9.0. The iodoacetyl group is an alkylating agent that reacts specifically with thiols (cysteines) at pH 7.5-8.5 to form a highly stable, irreversible thioether bond. While it reacts slower than a maleimide, the iodoacetyl group is less prone to hydrolysis. It is used for short-range, stable crosslinking of amines to thiols.

Molecular Weight

283.02

Purity

>95.0%

Formula

C₆H₆INO₄

Product Type

Heterobifunctional Crosslinkers

Group 1

NHS

Group 2

X (F, Cl, Br, I)

Form (20 deg.C)

Solid

Storage Condition

Store under inert gas, <0°C

Molecular Structure