Latex Particle-enhanced Turbidimetric Immunoassay (LETIA) Based Kits Development

Creative Biolabs is a world-class leader in the in vitro diagnostics (IVD) market and with years of proven expertise in IVD antibody and kits development, such as latex particle-enhanced turbidimetric immunoassay-based kits. Moreover, there are lots of professional scientists in biomedical fields and experienced staff operating experimental processes and machines to achieving clients’ requirements on their projects.

Latex Particle-enhanced Turbidimetric Immunoassay

LETIA Based Kits Development - Creative Biolabs

Routinely, the turbidimetric immunoassay is applicable to quantify serum concentration of proteins. Latex-particle enhancement of this type of assay, latex particle-enhanced turbidimetric immunoassay (LETIA or LTIA), has been primarily correlated with increased sensitivity. LETIA consists of latices coated with antibodies that react with a specific analyte that can be used to associate the triggered particle aggregation with the concentration of an analyte by means of a fast and easy detection, such as turbidimetry. This technique has a high degree of automation and is efficient in the measurement, as well as suitable for a large clinical analysis. Notably, the LETIA assay is much faster and convenient than enzyme-linked immunosorbent assay (ELISA) and suitable for clinical sample testing.

LETIA-based Kits Development

Covalent coupling of an antibody to a latex particle provides other advantages that are pertinent in the measurement of high analyte concentrations. A recent report describes the development of a latex-particle-enhanced and a non-enhanced turbidimetric assay to measure serum IgG through a normal antibody with IgG as a model analyte. Both methods reveal adequate analytical recovery and parallelism, meanwhile, the results compare well with that by rate nephelometry. The LETIA has equivalent sensitivity, interassay precision, and working range, but extremely greater calibration stability and signal change than the non-enhanced assay.

Schematic illustrating one possible mechanism of how antibody interferes with particle-enhanced turbidimetric immunoassays. Fig.1 Schematic illustrating one possible mechanism of how antibody interferes with particle-enhanced turbidimetric immunoassays. (Daly, M.L., 2008)

In most studies, the measurement of molecules in plasma, serum, and urine samples has been accomplished with either ELISA or immunoblotting systems. But these technologies have several inevitable drawbacks that make it difficult to obtain the reliable results and accurate evaluation of measurements.

In order to evaluate the analytical performance of experimental data, Creative Biolabs offers LETIA-based kits and related development services by our advanced experimental facilities and automatic platforms. Commercial kits can be applied in IVD fields for determining the diagnosis upon different medical conditions and promoting basic biological research and clinical practice. For more detailed information, please feel free to contact us.

Reference

  1. Daly, M.L., (2008). “A 58-year-old woman with abdominal symptoms and elevated C-reactive protein.” PLoS Med, 5(7), e149


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