The important role of disease-related protein detection in the fields of biological studies, clinical diagnostics, and medical treatment has driven the ever-increasing demand for developing simple, sensitive, highly selective, and cost-effective bioassays. Enzyme Linked Immune Sorbent Assay (ELISA) is considered as one of the most widely used techniques for protein detection due to its high sensitivity, high specificity, excellent accuracy, and practicality. Various methods and strategies based on different signal-transducer principles have been developed in Creative Biolabs. Our professional team is optimized to help you with high-quality and cost-effective testing service to make your project a success.
Colorimetric Immunoassay (CIA) Based Kits Development
Colorimetric immunoassay is the most commonly used method in immunoassays because of its simplicity, practicality, low cost and rapid/direct readout with the naked eye. Creative Biolabs has been devoted for the development of enzyme-based colorimetric immunoassays by using native enzyme labels as the signal amplification strategies, e.g. horseradish peroxidase (HRP), alkaline phosphatase (ALP) and β-galactosidase, due to their high catalytic activity, high specificity, mild reaction conditions, and easy conjugation to antibodies. Moreover, a single enzyme molecule can also catalytically transform many molecules of substrate into a product. Creative Biolabs also increases the sensitivity of enzyme-based colorimetric immunoassays by enhancing the ratio between enzyme molecule and target analyte.
Chemiluminescent Immunoassays (CLIA) Based Kits Development
Chemiluminescent immunoassay is a variation of the standard enzyme immunoassay (EIA), which is a biochemical technique used in immunology. They can also be used as diagnostic tools in medicine, as well as being used in several other different industries for various applications. This technique takes advantage of the basic immunology concept of antigen binding to specific antibodies, which uses enzymatically labeled antibodies and antigens to detect the desired small biological molecules.
Electrochemiluminescence Immunoassay (ECLIA) Based Kits Development
Creative Biolabs has been devoted for the development of highly sensitive, more flexible, quantitative, and easy-to-use electrochemiluminescence immunoassays-based kits. In electrochemiluminescence, electrochemically generated intermediates undergo a high degree of exergonic reaction to produce an electronically excited state and then emits light when relaxed to a lower energy level. This wavelength of emitted photons corresponds to the energy gap between these two states. ECL proved to be very useful in analytical applications as a highly sensitive and selective method. ECL provides a wider dynamic range, higher sensitivity, reduced matrix effects, and requires lower sample volumes, which allows for a broader assay.
Time Resolved Fluorescence Immunoassay (TRFIA) Based Kits Development
TRF relies on the use of very specific fluorescent molecules, the lanthanide. They have the unusual property of emitting over long periods of time (measured in milliseconds) after excitation when most standard fluorescent dyes (e.g. fluorescein) emit within a few nanoseconds of being excited. As an extension of fluorescence spectroscopy, time-resolved fluorescence assays are very robust (limited sensitivity to several types of assay interference) and are easily miniaturized.
Enzyme-linked Immunosorbent Spot (ELISpot) Based Kits Development
The ELISpot method was developed for the detection of antigen-specific antibody-secreting cells in a B cell ELISpot assay, a modification of the conventional sandwich ELISA immunoassay. It has proven to be among the most useful means available for monitoring cell-mediated immunity and has found clinical applications in the diagnosis of tuberculosis and the monitoring of graft tolerance or rejection in transplant patients. Cytokine Bead Arrays (CBA) and conventional ELISA assays can provide extremely useful information in certain contexts but lack the sensitivity and accuracy of ELISPOT for the detection and enumeration of rare antigen-specific cells.
Immuno-PCR Based Kits Development
Immuno-PCR is an antigen detection system in which polymerase chain reaction (PCR) is used to amplify labeled DNA fragments that specifically attach to antigen-antibody complexes. It combines the exponential signal amplification power of PCR with the versatility and flexibility of ELISA and offers great promise for its application in various aspects of biological and medical sciences, as well as clinical diagnostics and forensic medicine.
Protein Microarray Based Kits Development
Creative Biolabs has been devoted for the development of protein microarray-based kits, in which large numbers of proteins can be tracked in parallel. Protein microarray is a high-throughput method for tracking protein interactions and activities, determining their function, and determining function on a large scale.
ELISA utilizes antibodies to detect and quantify specific analytes such as peptides, proteins, and hormones. Creative Biolabs has significant experience in assay development with ELISA based kits. Our ELISA platform offers a sensitive and specific detection method that is also rapid and cost-effective. If you are interested, please feel free to contact us for more details.
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