Creative Biolabs has been devoted for the development of protein microarray-based kits, in which large numbers of proteins can be tracked in parallel. We also offer other ELISA kits for specific detection of disease-related proteins. Our professional team is optimized to help you with high-quality and cost-effective testing service to make your project a success.

How does it work?

Protein microarray is a high-throughput method for tracking protein interactions and activities, determining their function, and determining function on a large scale. The chip consists of a carrier surface such as a glass slide, nitrocellulose membrane, bead or microtiter plate, to which the capture protein array is bound. Probe molecules that are usually labeled with fluorescent dyes are added to the array. Any reaction between the probe and the immobilized protein emits a fluorescent signal and is read by a laser scanner. Protein microarrays are fast, automated, economical and highly sensitive, consuming small quantities of samples and reagents. The high-throughput technology behind protein microarrays is relatively easy to develop because it is based on technology developed for DNA microarrays, which have become the most widely used microarray. Protein array detection methods must provide a high signal and low background:

  • Fluorescence labeling. The most common and widely used method for detection for its highly sensitive, safe and compatible with readily available microarray laser scanners
  • Affinity tags
  • Photochemical tags
  • Radioisotope tags
  • Label free detection, such as carbon nanotubes, carbon nanowire sensors (where detection occurs via changes in conductance), surface plasmon resonance (SPR), and microelectromechanical system (MEMS) cantilevers

(a) Scheme typically used for allergy tests, infectious disease diagnosis and autoimmunity diagnostics. (b) Direct mode for protein detection. (c) Sandwich mode for protein detection. (d) Reverse-phase microarrays. Fig.1 Examples of different types of protein microarrays. (Cretich, M., 2014)

Protein Microarray Based Kits for IVD Development

Creative Biolabs has been devoted for the development of protein microarray-based kits, in which large numbers of proteins can be tracked in parallel. In vitro diagnostics includes detection of antigens and antibodies in blood samples; serum analysis to discover new disease biomarkers; monitoring of disease states and treatment responses in individualized diseases; monitoring of the environment and food. Digital bioassay is an example of diagnostics using protein microarrays. In this technique, a microwell array on a glass/polymer chip is seeded with magnetic beads (coated with fluorescently labeled antibodies), subjected to targeted antigens, and then characterized by microscopic counting of fluorescent wells. A cost-effective fabrication platform for the use of such microwell arrays (using OSTE polymers) have been demonstrated and biometric model systems have been successfully characterized.

Creative Biolabs is a world leader in the development of ELISA based kits and now offers protein microarray-based kits for specific detection of disease-related proteins. If you are interested in our ELISA kits development, please feel free to contact us for more details.

Reference

  1. Cretich, M., (2014). “Protein microarray technology: how far off is routine diagnostics?” Analyst 139(3): 528-542.


Online Inquiry

Name:
*Phone:
*E-mail Address:
*Service & Products Interested:
Project Description:
*Verification Code:
Please input "biolabs"(case insensitive) as verification code.

Contact Us

USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994
Fax: 1-631-207-8356
Email:

Follow us on:
Copyright ©2011 - 2019 Creative Biolabs.