As an extension of fluorescence spectroscopy, time-resolved fluorescence assays are very robust (limited sensitivity to several types of assay interference) and are easily miniaturized. Methods based on enzyme labels have been developed for time resolved fluorescence immunoassays in Creative Biolabs. We offer a wide range of ELISA kits for specific detection of disease-related proteins. Our professional team is optimized to help you with high-quality and cost-effective testing service to make your project a success.

How does it work?

Time-resolved spectroscopy uses spectroscopic techniques to study dynamic processes in materials or chemical compounds. With the help of pulsed lasers, the time scale can be studied as short as 10-16 seconds. This technique can be applied to any process which causes changes in the properties of the material. Time-resolved fluorescence spectroscopy is an extension of fluorescence spectroscopy. Here, the fluorescence of the sample is monitored as a function of time after being excited by the flash. The time resolution can be obtained in various ways (depending on the required sensitivity and time resolution), including:

  • With fast-detection electronics (nanoseconds and slower)
  • With Time Correlated Single Photon Counting, TCSPC (picoseconds and slower)
  • With a streak camera (picoseconds and slower)
  • With intensified CCD (ICCD) cameras (down to 200 picoseconds and slower)
  • With optical gating (femtoseconds-nanoseconds)

(A) Schematic diagram depicting the depolarization rates of free and nanocarrier bound fluorescent dye molecules; (B) Example of time-resolved fluorescence anisotropy curves r(t) of NR, free and loaded onto CMS nanocarriers (CMS/NR). Loaded drug: CMS/NR dissolved in an aqueous solution; released drug: CMS/NR in DMSO; only drug: NR in DMSO. Fig.1 Sensitivity of time-resolved fluorescence anisotropy curves r(t) for the conjugation state between nanocarrier and fluorescent drug (mimetic). (Boreham, A., 2016)

Advantages of Our Time Resolved Fluorescence Immunoassay (TRFIA) Platforms

Creative Biolabs has been devoted for the development of highly sensitive, more flexible, quantitative, and easy-to-use time resolved fluorescence immunoassay-based kits. TRF relies on the use of very specific fluorescent molecules, the lanthanide. They have the unusual property of emitting over long periods of time (measured in milliseconds) after excitation when most standard fluorescent dyes (e.g. fluorescein) emit within a few nanoseconds of being excited. As a result, the lanthanide element can be excited using a pulsed light source (such as a xenon flash lamp or a pulsed laser) and measured after the excitation pulse. This leads to a lower measurement background than the standard FI assay. The disadvantage is that the instruments and reagents are generally more expensive and the application must be compatible with the use of these very specific lanthanide dyes.

Creative Biolabs is a world leader in the development of ELISA based kits and offers a wide range of assay kits for specific detection of disease-related proteins. If you are interested in our ELISA kits development, please feel free to contact us for more details.

Reference

  1. Boreham, A., (2016). “Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine.” Molecules 22 (1).


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