With vast experience and leading-edge technology platforms, Creative Biolabs has developed an exhaustive portfolio to explore the functional mechanisms of antibody products. Our highly professional scientists have strong confidence in offering the best and most suitable service of cell proliferation assessment to worldwide clients.
Cell proliferation and viability act as a predominant indicator for evaluating therapeutic consequences and pharmacological safety during drug development. Antibodies, particularly, can elicit proliferation inhibition or cell death in multiple mechanisms of action, including antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), antibody-dependent cellular phagocytosis (ADCP), apoptosis, and other programmed cell death (PCD). In order to detect and evaluate the overall cytotoxic effects of your antibodies, Creative Biolabs brings out a panel of assay formats incorporation with high-throughput and high content analysis technology. We can also employ a full range of cell lines and well-validated disease models to assess proliferation alterations under more specific conditions.
Based on diverse research objectives, Creative Biolabs offers an integrated and versatile assay platform, which can generally be characterized as follows:
We have collected and optimized a series of classic in vitro proliferation assays. Since various signal pathways and cellular factors are involved in the proliferation process, we can provide multiple measure criteria including redox potential of cell population, ATP/ADP levels, integrity of cell membrane, activity of cellular enzymes, etc. These sophisticated molecular mechanism-oriented assays may shed valuable insight upon antibody functional perspectives in a simple, quick manner.
Fig. 1 Uncontrolled cell proliferation produces a high demand for oxygen and nutrients.1, 2
We have introduced a novel approach of real-time, label-free cell proliferation analysis using a superior microelectronic biosensor system. By real-time monitoring the electrical impedance, this system makes it feasible to obtain dynamic, quantitative data about cellular biological status, such as cell number, adhesion, viability and morphology changes. Therefore, it has revealed great potential in immune effector function analysis and immunomodulation studies with high sensitivity.
One major class of therapeutic antibodies are intended to kill target cells via immune effector cells, such as NK cell, T cells, and monocytes. Assays for such immunotherapy antibodies need to precisely detect target cell death while co-cultured with immune effector cells. Conventional proliferation tests thereby meet difficulties in setting two cell types apart. To address this issue, Creative Biolabs has developed a simple, non-radioactive and dye-free methodology to specifically detect proliferation changes of target cells in a co-culture system, which offers one powerful solution for analyzing immune-mediated cytotoxicity, i.e. ADCC and CDC.
Creative Biolabs innovatively brings out a 3D cell proliferation assay platform. In contrast to common 2D cell culture, this assay builds up a 3D environment in soft agar to mimic in vivo physiological conditions. In addition to proliferation, comprehensive information on multiple morphological parameters, such as cell adhesion, migration, and polarity, can also be acquired by this strategy.
Many reagents can cause growth inhibition via interfering with normal cell division. We offer integrated cell cycle analysis to in-depth detect cell cycle arrest at every specific point (G1-S-G2-M). Our strategies include:
We employ high content screening (HCS) using flow cytometry to robustly assess a large number of cells, either living or fixed, so as to generate statistically reliable results of cell cycle arrest in response to test antibodies.
Tumor Cell Proliferation Inhibition Assay Service
To better accelerate drug development, Creative Biolabs also developed an accurate and robust tumor cell proliferation inhibition assay service to support global clients in evaluating several immune checkpoint-related (such as PD-1, TIM-3, LAG-3, TIGIT, OX40, GITR, etc.) biologic or small molecules in inhibition of tumor cell proliferation.
Case Study
Fig.2 In vitro MCF-7 proliferation assay with samples.
Q1: How can we identify proliferating cells?
A1: Cell proliferation analysis is critical for cell differentiation and cancer research, and it is often used to assess chemical toxicity in the drug development process. We take advantage of methods for assessing cell proliferation including flow cytometry to measure DNA content, colorimetric assays to detect metabolic activity, and immunofluorescence to visualize cell cycle markers. In addition to these traditional approaches, we introduce innovative technologies such as live-cell imaging and 3D cell culturing to provide customers with more intuitive cell proliferation assay results.
Creative Biolabs is a reliable and trustworthy research partner who are willing to work closely with you step by step to facilitate your antibody developing projects. Notably, our seasoned scientists are also utterly specialized in tailoring custom assays according to different requirements. For more detailed information, please feel free to contact us or directly sent us an inquiry.
References
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