Creative Biolabs has made a long-term commitment to developing next-generation antibody therapies with our extensive expertise and world-class technology platforms. Our scientists currently are pleased to introduce our professional service of neutralization assays to our worldwide clients. Based on our integrated cell-based platform, we can provide services for evaluating the neutralizing functions of antibody candidates against different substances.
Neutralizing antibodies represent one important category of antibody therapeutics, which are intended to alter the bioactivity of target components through forming Ag-Ab complexes. In immunogenicity test, the presence of neutralizing antibodies, or anti-drug antibodies (ADA) often leads to abnormal PK behaviors or efficacy loss of corresponding drugs. On the other hand, antibodies that are capable of neutralizing harmful substances, i.e. viruses and cytokines, can be of great therapeutic potential. Currently, there have emerged plenty of antibody therapies based on neutralization mechanisms for the treatment of various infectious diseases, endocrine disorders, etc. Neutralization assays are specially designed to routinely validate and measure the therapeutic action of such antibodies.
Fig. 1 Various antibodies actions.1
Creative Biolabs offers antibody neutralization assays that are specifically designed to measure antibody neutralization potential against different targets, including but not limited to:
We can deal with purified antibodies or rough samples like antiserum, ascites, and tissue culture supernatants.
To validate whether the neutralization activities can translate into therapeutic effects, we must further investigate the biological changes of target cells in response to neutralizing agents. In fact, the cell-based format is strongly recommended by the regulatory department as it can best reveal the mechanism by which neutralizing antibodies exert their effects in vitro. After co-incubation of target cells for the indicated time, the antibody-target mixture will be transferred to target cell cultures in a 96-well plate. We apply appropriate controls to each group to ensure assay validity.
We provide various endpoints that can be tailored according to different research objectives. For pathogens, we usually identify its infectivity by the presence of a cytopathic effect (CPE) on target cells, or the reduction of proliferative effects, i.e. plaque reduction neutralization testing (PRNT), which has always been considered as a standard neutralization indicator for viruses. Besides, cell viability or cell lysis measurement like MTT, MST, trypan blue, is also another suitable choice. Our scientists also have extensive experience in designing customized readouts upon request.
We also provide ligand-binding assays to measure binding affinities as well as to evaluate neutralization efficacy in case cell-based format is difficult. Moreover, enzymatic activity analysis is also available if your antibody candidate is enzyme-neutralizing. With this highly sensitive, highly specific platform, Creative Biolabs can guarantee our clients with reliable and consistent outcomes to accelerate their diverse projects. Besides, our flexible platform is friendly to multiple assay formats and can be precisely tailored to meet all kinds of specific demands. For more detailed information, please feel free to contact us or directly sent us an inquiry.
Reference
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