With our advanced technology platform, Creative Biolabs is confident in providing in vitro cellular senescence analysis services to support our customers' projects.
Cellular senescence is one of the most fundamental characteristics of cell behavior, which is regarded to be extremely important in controlling cellular longevity both in vitro and in vivo. New information reveals that senescence may function as a tumor suppressor mechanism and links senescence bypass to the emergence of cancer. Thus, it is of great importance for drug development in cancer therapy.
Fig.1 General hallmarks of cellular senescence. (González-Gualda, 2021)
Increased expression of β-gal is a recognized marker of cell senescence. Our cellular senescence assay is mainly based on the detection of cell senescence by detecting the activity of β-gal with the help of various means. In this process, there are many factors that affect the final result, including cell culture, test articles treatment, and the usage of detection substrates. Creative Biolabs provides optimization of the whole experiment according to the customer's project.
Fig.2 Flowchart of senescence assay. (Creative Biolabs)
Powered by years of experience in cellular assays, Creative Biolabs provides in vitro cellular senescence assay services to suit your project. Our services have the following characteristics.
We have a wide range of human and mouse cell types for cell senescence experiments to support the drug development of cancer therapy.
Our cellular senescence assay services are suitable for both living cells and fixed cells, with shorter staining times, and can be quantitatively analyzed by flow cytometry.
Paper title: JAK inhibition alleviates the cellular senescence-associated secretory phenotype and frailty in old age
Cell type: Fat progenitor cells
Summary: In this study, the researchers studied cellular senescence and SASP using human primary preadipocytes and found that JAK inhibitors reduced SASP in preadipocytes and endothelial cells.
Results:
Fig.3 Senescent cells accumulate in adipose tissue as people age. (Xu, 2015)
Q1: Why do certain crystals grow after being in the air for a night?
A1: These salt crystals were created as a result of the solvent evaporating. We advise keeping the plate sealed while it is left overnight.
Q2: Can this procedure be used for frozen tissue sections?
A2: The tissues were placed into OCT after being frozen in liquid nitrogen. After fixing, washing, and staining, they were prepared for observation in a bright field.
If you are interested in our cellular senescence assay services, please feel free to contact us or directly sent us an inquiry.
References
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